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10.1186/s12879-021-06316-z http://scihub22266oqcxt.onion/10.1186/s12879-021-06316-z 34238234!8265726!34238234     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       BMC+Infect+Dis 2021 ; 21 (1): 665 Nephropedia Template TP {{tp|p=34238234|t=2021. Pooled sputum to optimise the efficiency and utility of rapid, point-of-care molecular SARS-CoV-2 testing |pdf=|usr=}}{{34238234}} gab.com Text Pooled sputum to optimise the efficiency and utility of rapid, point-of-care molecular SARS-CoV-2 testing JO: BMC Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=34238234 #FOAvip BACKGROUND: As SARS-CoV-2 testing expands, particularly to widespread asymptomatic testing, high sensitivity point-of-care PCR platforms may optimise potential benefits from pooling multiple patients' samples. METHOD: We tested patients and asymptomatic citizens for SARS-CoV-2, exploring the efficiency and utility of CovidNudge (i) for detection in individuals' sputum (compared to nasopharyngeal swabs), (ii) for detection in pooled sputum samples, and (iii) by modelling roll out scenarios for pooled sputum testing. RESULTS: Across 295 paired samples, we find no difference (p = 0.1236) in signal strength for sputum (mean amplified replicates (MAR) 25.2, standard deviation (SD) 14.2, range 0-60) compared to nasopharyngeal swabs (MAR 27.8, SD 12.4, range 6-56). At 10-sample pool size we find some drop in absolute strength of signal (individual sputum MAR 42.1, SD 11.8, range 13-60 vs. pooled sputum MAR 25.3, SD 14.6, range 1-54; p < 0.0001), but only marginal drop in sensitivity (51/53,96%). We determine a limit of detection of 250 copies/ml for an individual test, rising only four-fold to 1000copies/ml for a 10-sample pool. We find optimal pooled testing efficiency to be a 12-3-1-sample model, yet as prevalence increases, pool size should decrease; at 5% prevalence to maintain a 75% probability of negative first test, 5-sample pools are optimal. CONCLUSION: We describe for the first time the use of sequentially dipped sputum samples for rapid pooled point of care SARS-CoV-2 PCR testing. The potential to screen asymptomatic cohorts rapidly, at the point-of-care, with PCR, offers the potential to quickly identify and isolate positive individuals within a population "bubble". Twit Text FOAVip Pooled sputum to optimise the efficiency and utility of rapid, point-of-care molecular SARS-CoV-2 testing ????BMC Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=34238234 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34238234 #FOAvip English Wikipedia <ref>{{Cite pmid|34238234}}</ref> Pooled sputum to optimise the efficiency and utility of rapid, point-of-care molecular SARS-CoV-2 testing #MMPMID34238234 Burdett A; Toumazou C; Sahoo R; Mujan A; Hon TK; Bedzo-Nutakor J; Casali N; Karvela M; Sohbati M; Cooke GS; Davies GW; Moore LSP BMC Infect Dis 2021[Jul]; 21 (1): 665 PMID34238234show ga BACKGROUND: As SARS-CoV-2 testing expands, particularly to widespread asymptomatic testing, high sensitivity point-of-care PCR platforms may optimise potential benefits from pooling multiple patients' samples. METHOD: We tested patients and asymptomatic citizens for SARS-CoV-2, exploring the efficiency and utility of CovidNudge (i) for detection in individuals' sputum (compared to nasopharyngeal swabs), (ii) for detection in pooled sputum samples, and (iii) by modelling roll out scenarios for pooled sputum testing. RESULTS: Across 295 paired samples, we find no difference (p = 0.1236) in signal strength for sputum (mean amplified replicates (MAR) 25.2, standard deviation (SD) 14.2, range 0-60) compared to nasopharyngeal swabs (MAR 27.8, SD 12.4, range 6-56). At 10-sample pool size we find some drop in absolute strength of signal (individual sputum MAR 42.1, SD 11.8, range 13-60 vs. pooled sputum MAR 25.3, SD 14.6, range 1-54; p < 0.0001), but only marginal drop in sensitivity (51/53,96%). We determine a limit of detection of 250 copies/ml for an individual test, rising only four-fold to 1000copies/ml for a 10-sample pool. We find optimal pooled testing efficiency to be a 12-3-1-sample model, yet as prevalence increases, pool size should decrease; at 5% prevalence to maintain a 75% probability of negative first test, 5-sample pools are optimal. CONCLUSION: We describe for the first time the use of sequentially dipped sputum samples for rapid pooled point of care SARS-CoV-2 PCR testing. The potential to screen asymptomatic cohorts rapidly, at the point-of-care, with PCR, offers the potential to quickly identify and isolate positive individuals within a population "bubble". |*Point-of-Care Testing[MESH] |COVID-19 Testing/*methods[MESH] |COVID-19/*diagnosis/*virology[MESH] |Diagnostic Tests, Routine[MESH] |Humans[MESH] |Limit of Detection[MESH] |Nasopharynx/virology[MESH] |SARS-CoV-2/*isolation & purification[MESH] |Sensitivity and Specificity[MESH] |Sputum/*virology[MESH]Pooled sputum to optimise the efficiency and utility of rapid, point-o(epmc).pdf DeepDyve Pubget Overpricing