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10.1016/j.crmeth.2021.100011

http://scihub22266oqcxt.onion/10.1016/j.crmeth.2021.100011
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34235498!8114573!34235498
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suck abstract from ncbi


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pmid34235498      Cell+Rep+Methods 2021 ; 1 (2): 100011
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  • Rapid and accurate agglutination-based testing for SARS-CoV-2 antibodies #MMPMID34235498
  • Esmail S; Knauer MJ; Abdoh H; Voss C; Chin-Yee B; Stogios P; Seitova A; Hutchinson A; Yusifov F; Skarina T; Evdokimova E; Ackloo S; Lowes L; Hedley BD; Bhayana V; Chin-Yee I; Li SS
  • Cell Rep Methods 2021[Jun]; 1 (2): 100011 PMID34235498show ga
  • We have developed a rapid, accurate, and cost-effective serologic test for SARS-CoV-2 virus, which caused the COVID-19 pandemic, on the basis of antibody-dependent agglutination of antigen-coated latex particles. When validated using plasma samples that are positive or negative for SARS-CoV-2, the agglutination assay detected antibodies against the receptor-binding domain of the spike (S-RBD) or the nucleocapsid protein of SARS-CoV-2 with 100% specificity and approximately 98% sensitivity. Furthermore, we found that the strength of the S-RBD antibody response measured by the agglutination assay correlated with the efficiency of the plasma in blocking RBD binding to the angiotensin-converting enzyme 2 in a surrogate neutralization assay, suggesting that the agglutination assay might be used to identify individuals with virus-neutralizing antibodies. Intriguingly, we found that >92% of patients had detectable antibodies on the day of a positive viral RNA test, suggesting that the agglutination antibody test might complement RNA testing for the diagnosis of SARS-CoV-2 infection.
  • |*COVID-19/diagnosis[MESH]
  • |*SARS-CoV-2[MESH]
  • |Agglutination[MESH]
  • |Antibodies, Viral[MESH]
  • |Humans[MESH]


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