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10.1038/s41598-021-93439-7 http://scihub22266oqcxt.onion/10.1038/s41598-021-93439-7 34230585!8260644!34230585     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34230585&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\34230585.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Sci+Rep 2021 ; 11 (1): 13884 Nephropedia Template TP {{tp|p=34230585|t=2021. First molecular-based detection of SARS-CoV-2 virus in the field-collected houseflies |pdf=|usr=}}{{34230585}} gab.com Text First molecular-based detection of SARS-CoV-2 virus in the field-collected houseflies JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34230585 #FOAvip This is the first report of SARS-CoV-2 detection on field-collected Musca domestica housefly surface and tissue samples using the high-sensitive PCR assay which suggests the possible insect-borne transmission. The study was conducted in Shiraz city, southern Iran, in May and Jun 2020. Adult flies were sampled at the outdoor areas of two hospitals treating COVID-19 patients. Fly samples were first washed twice to remove the insect surface attached to SARS-CoV-2 virions. After that, the disinfected fly samples were homogenized. Fly surface washout and homogenate samples were tested using Taq Man real-time PCR assay for the SARS-CoV-2 virus. In a total of 156 houseflies, 75% of samples from the body washout samples were positive for SARS-CoV-2. Strikingly, 37% of the homogenized specimens were positive for the SARS-CoV-2, suggesting the possible infection of the insects or uptake of the virion to the insect metabolism. The other possibility is the houseflies up took the blood or blood fluids of the patients and the RNA of the SARS-CoV-2 survived in the insect body without replicating. Our preliminary findings suggest that the houseflies could transmit SARS-CoV-2 as a mechanical or biological vector especially during the warm seasons while increasing the population and activity of houseflies. Twit Text FOAVip First molecular-based detection of SARS-CoV-2 virus in the field-collected houseflies ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34230585 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34230585 #FOAvip English Wikipedia <ref>{{Cite pmid|34230585}}</ref> First molecular-based detection of SARS-CoV-2 virus in the field-collected houseflies #MMPMID34230585 Soltani A; Jamalidoust M; Hosseinpour A; Vahedi M; Ashraf H; Yousefinejad S Sci Rep 2021[Jul]; 11 (1): 13884 PMID34230585show ga This is the first report of SARS-CoV-2 detection on field-collected Musca domestica housefly surface and tissue samples using the high-sensitive PCR assay which suggests the possible insect-borne transmission. The study was conducted in Shiraz city, southern Iran, in May and Jun 2020. Adult flies were sampled at the outdoor areas of two hospitals treating COVID-19 patients. Fly samples were first washed twice to remove the insect surface attached to SARS-CoV-2 virions. After that, the disinfected fly samples were homogenized. Fly surface washout and homogenate samples were tested using Taq Man real-time PCR assay for the SARS-CoV-2 virus. In a total of 156 houseflies, 75% of samples from the body washout samples were positive for SARS-CoV-2. Strikingly, 37% of the homogenized specimens were positive for the SARS-CoV-2, suggesting the possible infection of the insects or uptake of the virion to the insect metabolism. The other possibility is the houseflies up took the blood or blood fluids of the patients and the RNA of the SARS-CoV-2 survived in the insect body without replicating. Our preliminary findings suggest that the houseflies could transmit SARS-CoV-2 as a mechanical or biological vector especially during the warm seasons while increasing the population and activity of houseflies. |*Molecular Diagnostic Techniques[MESH] |Animals[MESH] |Houseflies/*virology[MESH] |Humans[MESH] |Insect Vectors/*virology[MESH] |Iran[MESH] |Real-Time Polymerase Chain Reaction/methods[MESH] |SARS-CoV-2/*pathogenicity[MESH]First molecular-based detection of SARS-CoV-2 virus in the field-colle(epmc).pdf DeepDyve Pubget Overpricing