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10.1007/s12020-021-02807-w http://scihub22266oqcxt.onion/10.1007/s12020-021-02807-w 34224085!8256224!34224085     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34224085&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Endocrine 2021 ; 74 (3): 638-645 Nephropedia Template TP {{tp|p=34224085|t=2021. Modulation of ACE-2 mRNA by inflammatory cytokines in human thyroid cells: a pilot study |pdf=|usr=}}{{34224085}} gab.com Text Modulation of ACE-2 mRNA by inflammatory cytokines in human thyroid cells: a pilot study JO: Endocrine http://www.kidney.de/pget.php?&tw=1&pmid=34224085 #FOAvip INTRODUCTION: Angiotensin-converting-enzyme-2 (ACE-2) was demonstrated to be the receptor for cellular entry of SARS-CoV-2. ACE-2 mRNA was identified in several human tissues and recently also in thyroid cells in vitro. PURPOSE: Aim of the present study was to investigate the effect of pro-inflammatory cytokines on the ACE-2 mRNA levels in human thyroid cells in primary cultures. METHODS: Primary thyroid cell cultures were treated with IFN-gamma and TNF-alpha alone or in combination for 24 h. ACE-2 mRNA levels were measured by RT-PCR. As a control, the levels of IFN-gamma inducible chemokine (CXCL10) were measured in the respective cell culture supernatants. RESULTS: The mean levels of ACE-2 mRNA increased after treatment with IFN-gamma and TNF-alpha in all the thyroid cell preparations, while the combination treatment did not consistently synergically increase ACE-2-mRNA. At difference, CXCL10 was consistently increased by IFN-gamma and synergically further increased by the combination treatment with IFN-gamma + TNF-alpha, with respect to IFN-gamma alone. CONCLUSIONS: The results of the present study show that IFN-gamma and, to a lesser extent TNF-alpha consistently increase ACE-2 mRNA levels in NHT primary cultures. More interestingly, the combined stimulation (proven to be effective according to the synergic effect registered for CXCL10) produces different responses in terms of ACE-2 mRNA modulation. These results would suggest that elevated levels of pro-inflammatory cytokines could facilitate the entering of the virus in cells by further increasing ACE-2 expression and/or account for the different degree of severity of SARS-COV-2 infection. This hypothesis deserves to be confirmed by further specific studies. Twit Text FOAVip Modulation of ACE-2 mRNA by inflammatory cytokines in human thyroid cells: a pilot study ????Endocrine http://www.kidney.de/pget.php?&tw=1&pmid=34224085 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34224085 #FOAvip English Wikipedia <ref>{{Cite pmid|34224085}}</ref> Modulation of ACE-2 mRNA by inflammatory cytokines in human thyroid cells: a pilot study #MMPMID34224085 Coperchini F; Ricci G; Croce L; Denegri M; Ruggiero R; Villani L; Magri F; Chiovato L; Rotondi M Endocrine 2021[Dec]; 74 (3): 638-645 PMID34224085show ga INTRODUCTION: Angiotensin-converting-enzyme-2 (ACE-2) was demonstrated to be the receptor for cellular entry of SARS-CoV-2. ACE-2 mRNA was identified in several human tissues and recently also in thyroid cells in vitro. PURPOSE: Aim of the present study was to investigate the effect of pro-inflammatory cytokines on the ACE-2 mRNA levels in human thyroid cells in primary cultures. METHODS: Primary thyroid cell cultures were treated with IFN-gamma and TNF-alpha alone or in combination for 24 h. ACE-2 mRNA levels were measured by RT-PCR. As a control, the levels of IFN-gamma inducible chemokine (CXCL10) were measured in the respective cell culture supernatants. RESULTS: The mean levels of ACE-2 mRNA increased after treatment with IFN-gamma and TNF-alpha in all the thyroid cell preparations, while the combination treatment did not consistently synergically increase ACE-2-mRNA. At difference, CXCL10 was consistently increased by IFN-gamma and synergically further increased by the combination treatment with IFN-gamma + TNF-alpha, with respect to IFN-gamma alone. CONCLUSIONS: The results of the present study show that IFN-gamma and, to a lesser extent TNF-alpha consistently increase ACE-2 mRNA levels in NHT primary cultures. More interestingly, the combined stimulation (proven to be effective according to the synergic effect registered for CXCL10) produces different responses in terms of ACE-2 mRNA modulation. These results would suggest that elevated levels of pro-inflammatory cytokines could facilitate the entering of the virus in cells by further increasing ACE-2 expression and/or account for the different degree of severity of SARS-COV-2 infection. This hypothesis deserves to be confirmed by further specific studies. |*COVID-19[MESH] |*Thyroid Gland[MESH] |Angiotensin-Converting Enzyme 2[MESH] |Cytokines[MESH] |Humans[MESH] |Pilot Projects[MESH] |RNA, Messenger[MESH] |SARS-CoV-2[MESH]Modulation of ACE-2 mRNA by inflammatory cytokines in human thyroid ce(epmc).pdf DeepDyve Pubget Overpricing