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10.1016/j.diagmicrobio.2021.115387 http://scihub22266oqcxt.onion/10.1016/j.diagmicrobio.2021.115387 34218165!9761777!34218165     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34218165&cmd=llinks): Failed to open stream: HTTP request failed! 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Evaluation of PCR cycle threshold values by patient population with the quidel lyra SARS-CoV-2 assay |pdf=|usr=}}{{34218165}} gab.com Text Evaluation of PCR cycle threshold values by patient population with the quidel lyra SARS-CoV-2 assay JO: Diagn Microbiol Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=34218165 #FOAvip The Lyra SARS-CoV-2 assay was the primary method for molecular testing performed at Barnes-Jewish Healthcare System in St. Louis, Missouri during the initial COVID-19 surge from mid-March to late-April 2020. We performed a retrospective analysis of 1,043 positive Lyra SARS-CoV-2 results during these 36 days to investigate associations between cycle threshold (C(T)) value and patient characteristics. Total RNA were extracted from NP or OP swabs using either the EasyMag or KingFisher automated extraction systems and quantified with RotorGene Q (Qiagen) or Applied Biosystems 7500 Fast Dx thermocyclers respectively. Notably, we found lower a significant median lower C(T) for samples tested on the KingFisher-ABI 7500 fastDX (KF/ABI) system compared to the EasyMag/RotorGene (EM/RGQ) platform. Since 77.5% of our tests were ran on the EM/RGQ pipeline we then perform additional analysis on these values and found that C (T) values in outpatient care settings compared to samples obtained in the emergency department or inpatient had significantly lower C (T) values. These collective findings suggests a difference in viral load amongst various patient populations. Twit Text FOAVip Evaluation of PCR cycle threshold values by patient population with the quidel lyra SARS-CoV-2 assay ????Diagn Microbiol Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=34218165 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34218165 #FOAvip English Wikipedia <ref>{{Cite pmid|34218165}}</ref> Evaluation of PCR cycle threshold values by patient population with the quidel lyra SARS-CoV-2 assay #MMPMID34218165 Potter RF; Abro B; Eby CS; Burnham CD; Anderson NW; Parikh BA Diagn Microbiol Infect Dis 2021[Oct]; 101 (2): 115387 PMID34218165show ga The Lyra SARS-CoV-2 assay was the primary method for molecular testing performed at Barnes-Jewish Healthcare System in St. Louis, Missouri during the initial COVID-19 surge from mid-March to late-April 2020. We performed a retrospective analysis of 1,043 positive Lyra SARS-CoV-2 results during these 36 days to investigate associations between cycle threshold (C(T)) value and patient characteristics. Total RNA were extracted from NP or OP swabs using either the EasyMag or KingFisher automated extraction systems and quantified with RotorGene Q (Qiagen) or Applied Biosystems 7500 Fast Dx thermocyclers respectively. Notably, we found lower a significant median lower C(T) for samples tested on the KingFisher-ABI 7500 fastDX (KF/ABI) system compared to the EasyMag/RotorGene (EM/RGQ) platform. Since 77.5% of our tests were ran on the EM/RGQ pipeline we then perform additional analysis on these values and found that C (T) values in outpatient care settings compared to samples obtained in the emergency department or inpatient had significantly lower C (T) values. These collective findings suggests a difference in viral load amongst various patient populations. |Age Factors[MESH] |Ambulatory Care/statistics & numerical data[MESH] |COVID-19 Nucleic Acid Testing/*statistics & numerical data[MESH] |COVID-19/*diagnosis[MESH] |Emergency Medical Services/statistics & numerical data[MESH] |Hospitalization/statistics & numerical data[MESH] |Humans[MESH] |Missouri/epidemiology[MESH] |Pharynx/virology[MESH] |Retrospective Studies[MESH] |SARS-CoV-2/genetics/*isolation & purification[MESH]Evaluation of PCR cycle threshold values by patient population with th(epmc).pdf DeepDyve Pubget Overpricing