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10.1111/trf.16580 http://scihub22266oqcxt.onion/10.1111/trf.16580 34216156!8440373!34216156     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Transfusion 2021 ; 61 (9): 2658-2667 Nephropedia Template TP {{tp|p=34216156|t=2021. Assessment of serological assays for identifying high titer convalescent plasma |pdf=|usr=}}{{34216156}} gab.com Text Assessment of serological assays for identifying high titer convalescent plasma JO: Transfusion http://www.kidney.de/pget.php?&tw=1&pmid=34216156 #FOAvip BACKGROUND: The COVID-19 pandemic has been accompanied by the largest mobilization of therapeutic convalescent plasma (CCP) in over a century. Initial identification of high titer units was based on dose-response data using the Ortho VITROS IgG assay. The proliferation of severe acute respiratory syndrome coronavirus 2 serological assays and non-uniform application has led to uncertainty about their interrelationships. The purpose of this study was to establish correlations and analogous cutoffs between multiple serological assays. METHODS: We compared the Ortho, Abbott, Roche, an anti-spike (S) ELISA, and a virus neutralization assay. Relationships relative to FDA-approved cutoffs under the CCP emergency use authorization were identified in convalescent plasma from a cohort of 79 donors from April 2020. RESULTS: Relative to the neutralization assay, the spearman r value of the Ortho Clinical, Abbott, Roche, anti-S ELISA assays was 0.65, 0.59, 0.45, and 0.76, respectively. The best correlative index for establishing high-titer units was 3.87 signal-to-cutoff (S/C) for the Abbott, 13.82 cutoff index for the Roche, 1:1412 for the anti-S ELISA, 1:219 by the neutralization assay, and 15.9 S/C by the Ortho Clinical assay. The overall agreement using derived cutoffs compared to a neutralizing titer of 1:250 was 78.5% for Abbott, 74.7% for Roche, 83.5% for the anti-S ELISA, and 78.5% for Ortho Clinical. DISCUSSION: Assays based on antibodies against the nucleoprotein were positively associated with neutralizing titers and the Ortho assay, although their ability to distinguish FDA high-titer specimens was imperfect. The resulting relationships help reconcile results from the large body of serological data generated during the COVID-19 pandemic. Twit Text FOAVip Assessment of serological assays for identifying high titer convalescent plasma ????Transfusion http://www.kidney.de/pget.php?&tw=1&pmid=34216156 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34216156 #FOAvip English Wikipedia <ref>{{Cite pmid|34216156}}</ref> Assessment of serological assays for identifying high titer convalescent plasma #MMPMID34216156 Farnsworth CW; Case JB; Hock K; Chen RE; O'Halloran JA; Presti R; Goss CW; Rauseo AM; Ellebedy A; Theel ES; Diamond MS; Henderson JP Transfusion 2021[Sep]; 61 (9): 2658-2667 PMID34216156show ga BACKGROUND: The COVID-19 pandemic has been accompanied by the largest mobilization of therapeutic convalescent plasma (CCP) in over a century. Initial identification of high titer units was based on dose-response data using the Ortho VITROS IgG assay. The proliferation of severe acute respiratory syndrome coronavirus 2 serological assays and non-uniform application has led to uncertainty about their interrelationships. The purpose of this study was to establish correlations and analogous cutoffs between multiple serological assays. METHODS: We compared the Ortho, Abbott, Roche, an anti-spike (S) ELISA, and a virus neutralization assay. Relationships relative to FDA-approved cutoffs under the CCP emergency use authorization were identified in convalescent plasma from a cohort of 79 donors from April 2020. RESULTS: Relative to the neutralization assay, the spearman r value of the Ortho Clinical, Abbott, Roche, anti-S ELISA assays was 0.65, 0.59, 0.45, and 0.76, respectively. The best correlative index for establishing high-titer units was 3.87 signal-to-cutoff (S/C) for the Abbott, 13.82 cutoff index for the Roche, 1:1412 for the anti-S ELISA, 1:219 by the neutralization assay, and 15.9 S/C by the Ortho Clinical assay. The overall agreement using derived cutoffs compared to a neutralizing titer of 1:250 was 78.5% for Abbott, 74.7% for Roche, 83.5% for the anti-S ELISA, and 78.5% for Ortho Clinical. DISCUSSION: Assays based on antibodies against the nucleoprotein were positively associated with neutralizing titers and the Ortho assay, although their ability to distinguish FDA high-titer specimens was imperfect. The resulting relationships help reconcile results from the large body of serological data generated during the COVID-19 pandemic. |Adult[MESH] |Aged[MESH] |Antibodies, Neutralizing/blood/immunology[MESH] |Antibodies, Viral/blood/*immunology[MESH] |COVID-19 Serological Testing[MESH] |COVID-19 Serotherapy[MESH] |COVID-19/blood/*epidemiology/*immunology/therapy[MESH] |Comorbidity[MESH] |Enzyme-Linked Immunosorbent Assay[MESH] |Female[MESH] |Humans[MESH] |Immunization, Passive[MESH] |Immunoglobulin G/blood/immunology[MESH] |Male[MESH] |Middle Aged[MESH] |ROC Curve[MESH] |SARS-CoV-2/*immunology[MESH] |Seroepidemiologic Studies[MESH]Assessment of serological assays for identifying high titer convalesce(epmc).pdf DeepDyve Pubget Overpricing