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10.1016/j.talanta.2021.122532 http://scihub22266oqcxt.onion/10.1016/j.talanta.2021.122532 34215035!8133803!34215035     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Talanta 2021 ; 233 (ä): 122532 Nephropedia Template TP {{tp|p=34215035|t=2021. SARS-CoV-2 spike protein detection through a plasmonic D-shaped plastic optical fiber aptasensor |pdf=|usr=}}{{34215035}} gab.com Text SARS-CoV-2 spike protein detection through a plasmonic D-shaped plastic optical fiber aptasensor JO: Talanta http://www.kidney.de/pget.php?&tw=1&pmid=34215035 #FOAvip A specific aptameric sequence has been immobilized on short polyethyleneglycol (PEG) interface on gold nano-film deposited on a D-shaped plastic optical fiber (POFs) probe, and the protein binding has been monitored exploiting the very sensitive surface plasmon resonance (SPR) phenomenon. The receptor-binding domain (RBD) of the SARS-CoV-2 spike glycoprotein has been specifically used to develop an aptasensor. Surface analysis techniques coupled to fluorescence microscopy and plasmonic analysis have been utilized to characterize the biointerface. Spanning a wide protein range (25 / 1000 nM), the SARS-Cov-2 spike protein was detected with a Limit of Detection (LoD) of about 37 nM. Different interferents (BSA, AH1N1 hemagglutinin protein and MERS spike protein) have been tested confirming the specificity of our aptasensor. Finally, a preliminary test in diluted human serum encouraged its application in a point-of-care device, since POF-based aptasensor represent a potentially low-cost compact biosensor, characterized by a rapid response, a small size and could be an ideal laboratory portable diagnostic tool. Twit Text FOAVip SARS-CoV-2 spike protein detection through a plasmonic D-shaped plastic optical fiber aptasensor ????Talanta http://www.kidney.de/pget.php?&tw=1&pmid=34215035 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34215035 #FOAvip English Wikipedia <ref>{{Cite pmid|34215035}}</ref> SARS-CoV-2 spike protein detection through a plasmonic D-shaped plastic optical fiber aptasensor #MMPMID34215035 Cennamo N; Pasquardini L; Arcadio F; Lunelli L; Vanzetti L; Carafa V; Altucci L; Zeni L Talanta 2021[Oct]; 233 (ä): 122532 PMID34215035show ga A specific aptameric sequence has been immobilized on short polyethyleneglycol (PEG) interface on gold nano-film deposited on a D-shaped plastic optical fiber (POFs) probe, and the protein binding has been monitored exploiting the very sensitive surface plasmon resonance (SPR) phenomenon. The receptor-binding domain (RBD) of the SARS-CoV-2 spike glycoprotein has been specifically used to develop an aptasensor. Surface analysis techniques coupled to fluorescence microscopy and plasmonic analysis have been utilized to characterize the biointerface. Spanning a wide protein range (25 / 1000 nM), the SARS-Cov-2 spike protein was detected with a Limit of Detection (LoD) of about 37 nM. Different interferents (BSA, AH1N1 hemagglutinin protein and MERS spike protein) have been tested confirming the specificity of our aptasensor. Finally, a preliminary test in diluted human serum encouraged its application in a point-of-care device, since POF-based aptasensor represent a potentially low-cost compact biosensor, characterized by a rapid response, a small size and could be an ideal laboratory portable diagnostic tool. |*COVID-19[MESH] |*Optical Fibers[MESH] |Humans[MESH] |Plastics[MESH] |SARS-CoV-2[MESH]SARS-CoV-2 spike protein detection through a plasmonic D-shaped plasti(epmc).pdf DeepDyve Pubget Overpricing