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10.1002/jcla.23888 http://scihub22266oqcxt.onion/10.1002/jcla.23888 34213803!8418467!34213803     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Lab+Anal 2021 ; 35 (9): e23888 Nephropedia Template TP {{tp|p=34213803|t=2021. The Hologic Aptima SARS-CoV-2 assay enables high ratio pooling saving reagents and improving turnaround time |pdf=|usr=}}{{34213803}} gab.com Text The Hologic Aptima SARS-CoV-2 assay enables high ratio pooling saving reagents and improving turnaround time JO: J Clin Lab Anal http://www.kidney.de/pget.php?&tw=1&pmid=34213803 #FOAvip BACKGROUND: The Hologic Aptima TMA SARS-CoV-2 assay was employed to test pooled nasopharyngeal (NP) samples to evaluate the performance of pooled sample testing and characterize variables influencing results. METHODS: Results on 1033 previously tested NP samples were retrieved to characterize the relative light units (RLU) of SARS-CoV-2-positive samples in the tested population. The pooling strategy of combining 10 SARS-CoV-2 samples into one pool (10/1) was used in this study. The results were compared with neat sample testing using the same Aptima TMA SARS-CoV-2 assay and also the CDC RT-PCR and the Cepheid SARS-CoV-2 assays. RESULTS: The Aptima assay compares favorably with both CDC RT-PCR and the Cepheid SARS-CoV-2 assays. Once samples are pooled 10 to 1 as in our experiments, the resulting signal strength of the assay suffers. A divide opens between pools assembled from strong-positive versus only weak-positive samples. Pools of the former can be reliably detected with positive percent agreement (PPA) of 95.2%, while pools of the latter are frequently misclassified as negative with PPA of 40%. When the weak-positive samples with kRLU value lower than 1012 constitute 3.4% of the total sample profile, the assay PPA approaches 93.4% suggesting that 10/1 pooled sample testing by the Aptima assay is an effective screening tool for SARS-CoV-2. CONCLUSION: Performing pooled testing, one should monitor the weak positives with kRLU lower than 1012 or quantification cycle (Cq) value higher than 35 on an ongoing basis and adjust pooling approaches to avoid reporting false negatives. Twit Text FOAVip The Hologic Aptima SARS-CoV-2 assay enables high ratio pooling saving reagents and improving turnaround time ????J Clin Lab Anal http://www.kidney.de/pget.php?&tw=1&pmid=34213803 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34213803 #FOAvip English Wikipedia <ref>{{Cite pmid|34213803}}</ref> The Hologic Aptima SARS-CoV-2 assay enables high ratio pooling saving reagents and improving turnaround time #MMPMID34213803 Newsom K; Zhang Y; Chamala S; Martinez K; Clare-Salzler M; Starostik P J Clin Lab Anal 2021[Sep]; 35 (9): e23888 PMID34213803show ga BACKGROUND: The Hologic Aptima TMA SARS-CoV-2 assay was employed to test pooled nasopharyngeal (NP) samples to evaluate the performance of pooled sample testing and characterize variables influencing results. METHODS: Results on 1033 previously tested NP samples were retrieved to characterize the relative light units (RLU) of SARS-CoV-2-positive samples in the tested population. The pooling strategy of combining 10 SARS-CoV-2 samples into one pool (10/1) was used in this study. The results were compared with neat sample testing using the same Aptima TMA SARS-CoV-2 assay and also the CDC RT-PCR and the Cepheid SARS-CoV-2 assays. RESULTS: The Aptima assay compares favorably with both CDC RT-PCR and the Cepheid SARS-CoV-2 assays. Once samples are pooled 10 to 1 as in our experiments, the resulting signal strength of the assay suffers. A divide opens between pools assembled from strong-positive versus only weak-positive samples. Pools of the former can be reliably detected with positive percent agreement (PPA) of 95.2%, while pools of the latter are frequently misclassified as negative with PPA of 40%. When the weak-positive samples with kRLU value lower than 1012 constitute 3.4% of the total sample profile, the assay PPA approaches 93.4% suggesting that 10/1 pooled sample testing by the Aptima assay is an effective screening tool for SARS-CoV-2. CONCLUSION: Performing pooled testing, one should monitor the weak positives with kRLU lower than 1012 or quantification cycle (Cq) value higher than 35 on an ongoing basis and adjust pooling approaches to avoid reporting false negatives. |COVID-19 Testing/instrumentation/*methods[MESH] |COVID-19/*diagnosis/virology[MESH] |False Negative Reactions[MESH] |Humans[MESH] |Mass Screening/methods[MESH] |Nasopharynx/*virology[MESH] |Reproducibility of Results[MESH] |Reverse Transcriptase Polymerase Chain Reaction[MESH]The Hologic Aptima SARS-CoV-2 assay enables high ratio pooling saving (epmc).pdf DeepDyve Pubget Overpricing