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10.1038/s41551-021-00754-5 http://scihub22266oqcxt.onion/10.1038/s41551-021-00754-5 34211145!10810734!34211145     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Biomed+Eng 2021 ; 5 (7): 657-665 Nephropedia Template TP {{tp|p=34211145|t=2021. Massively scaled-up testing for SARS-CoV-2 RNA via next-generation sequencing of pooled and barcoded nasal and saliva samples |pdf=|usr=}}{{34211145}} gab.com Text Massively scaled-up testing for SARS-CoV-2 RNA via next-generation sequencing of pooled and barcoded nasal and saliva samples JO: Nat Biomed Eng http://www.kidney.de/pget.php?&tw=1&pmid=34211145 #FOAvip Frequent and widespread testing of members of the population who are asymptomatic for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for the mitigation of the transmission of the virus. Despite the recent increases in testing capacity, tests based on quantitative polymerase chain reaction (qPCR) assays cannot be easily deployed at the scale required for population-wide screening. Here, we show that next-generation sequencing of pooled samples tagged with sample-specific molecular barcodes enables the testing of thousands of nasal or saliva samples for SARS-CoV-2 RNA in a single run without the need for RNA extraction. The assay, which we named SwabSeq, incorporates a synthetic RNA standard that facilitates end-point quantification and the calling of true negatives, and that reduces the requirements for automation, purification and sample-to-sample normalization. We used SwabSeq to perform 80,000 tests, with an analytical sensitivity and specificity comparable to or better than traditional qPCR tests, in less than two months with turnaround times of less than 24 h. SwabSeq could be rapidly adapted for the detection of other pathogens. Twit Text FOAVip Massively scaled-up testing for SARS-CoV-2 RNA via next-generation sequencing of pooled and barcoded nasal and saliva samples ????Nat Biomed Eng http://www.kidney.de/pget.php?&tw=1&pmid=34211145 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34211145 #FOAvip English Wikipedia <ref>{{Cite pmid|34211145}}</ref> Massively scaled-up testing for SARS-CoV-2 RNA via next-generation sequencing of pooled and barcoded nasal and saliva samples #MMPMID34211145 Bloom JS; Sathe L; Munugala C; Jones EM; Gasperini M; Lubock NB; Yarza F; Thompson EM; Kovary KM; Park J; Marquette D; Kay S; Lucas M; Love T; Sina Booeshaghi A; Brandenberg OF; Guo L; Boocock J; Hochman M; Simpkins SW; Lin I; LaPierre N; Hong D; Zhang Y; Oland G; Choe BJ; Chandrasekaran S; Hilt EE; Butte MJ; Damoiseaux R; Kravit C; Cooper AR; Yin Y; Pachter L; Garner OB; Flint J; Eskin E; Luo C; Kosuri S; Kruglyak L; Arboleda VA Nat Biomed Eng 2021[Jul]; 5 (7): 657-665 PMID34211145show ga Frequent and widespread testing of members of the population who are asymptomatic for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for the mitigation of the transmission of the virus. Despite the recent increases in testing capacity, tests based on quantitative polymerase chain reaction (qPCR) assays cannot be easily deployed at the scale required for population-wide screening. Here, we show that next-generation sequencing of pooled samples tagged with sample-specific molecular barcodes enables the testing of thousands of nasal or saliva samples for SARS-CoV-2 RNA in a single run without the need for RNA extraction. The assay, which we named SwabSeq, incorporates a synthetic RNA standard that facilitates end-point quantification and the calling of true negatives, and that reduces the requirements for automation, purification and sample-to-sample normalization. We used SwabSeq to perform 80,000 tests, with an analytical sensitivity and specificity comparable to or better than traditional qPCR tests, in less than two months with turnaround times of less than 24 h. SwabSeq could be rapidly adapted for the detection of other pathogens. |High-Throughput Nucleotide Sequencing[MESH] |Humans[MESH] |RNA, Viral/*genetics[MESH] |SARS-CoV-2/genetics/*pathogenicity[MESH] |Saliva/*virology[MESH]Massively scaled-up testing for SARS-CoV-2 RNA via next-generation seq(epmc).pdf DeepDyve Pubget Overpricing