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10.3390/foods10071503 http://scihub22266oqcxt.onion/10.3390/foods10071503 34209659!8304378!34209659     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34209659&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Foods 2021 ; 10 (7): ? Nephropedia Template TP {{tp|p=34209659|t=2021. Potential In Vitro Inhibition of Selected Plant Extracts against SARS-CoV-2 Chymotripsin-Like Protease (3CL(Pro)) Activity |pdf=|usr=}}{{34209659}} gab.com Text Potential In Vitro Inhibition of Selected Plant Extracts against SARS-CoV-2 Chymotripsin-Like Protease (3CL(Pro)) Activity JO: Foods http://www.kidney.de/pget.php?&tw=1&pmid=34209659 #FOAvip Antiviral treatments inhibiting Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replication may represent a strategy complementary to vaccination to fight the ongoing Coronavirus disease 19 (COVID-19) pandemic. Molecules or extracts inhibiting the SARS-CoV-2 chymotripsin-like protease (3CL(Pro)) could contribute to reducing or suppressing SARS-CoV-2 replication. Using a targeted approach, we identified 17 plant products that are included in current and traditional cuisines as promising inhibitors of SARS-CoV-2 3CL(Pro) activity. Methanolic extracts were evaluated in vitro for inhibition of SARS-CoV-2 3CL(Pro) activity using a quenched fluorescence resonance energy transfer (FRET) assay. Extracts from turmeric (Curcuma longa) rhizomes, mustard (Brassica nigra) seeds, and wall rocket (Diplotaxis erucoides subsp. erucoides) at 500 microg mL(-1) displayed significant inhibition of the 3CL(Pro) activity, resulting in residual protease activities of 0.0%, 9.4%, and 14.9%, respectively. Using different extract concentrations, an IC(50) value of 15.74 microg mL(-1) was calculated for turmeric extract. Commercial curcumin inhibited the 3CL(Pro) activity, but did not fully account for the inhibitory effect of turmeric rhizomes extracts, suggesting that other components of the turmeric extract must also play a main role in inhibiting the 3CL(Pro) activity. Sinigrin, a major glucosinolate present in mustard seeds and wall rocket, did not have relevant 3CL(Pro) inhibitory activity; however, its hydrolysis product allyl isothiocyanate had an IC(50) value of 41.43 microg mL(-1). The current study identifies plant extracts and molecules that can be of interest in the search for treatments against COVID-19, acting as a basis for future chemical, in vivo, and clinical trials. Twit Text FOAVip Potential In Vitro Inhibition of Selected Plant Extracts against SARS-CoV-2 Chymotripsin-Like Protease (3CL(Pro)) Activity ????Foods http://www.kidney.de/pget.php?&tw=1&pmid=34209659 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34209659 #FOAvip English Wikipedia <ref>{{Cite pmid|34209659}}</ref> Potential In Vitro Inhibition of Selected Plant Extracts against SARS-CoV-2 Chymotripsin-Like Protease (3CL(Pro)) Activity #MMPMID34209659 Guijarro-Real C; Plazas M; Rodriguez-Burruezo A; Prohens J; Fita A Foods 2021[Jun]; 10 (7): ? PMID34209659show ga Antiviral treatments inhibiting Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) replication may represent a strategy complementary to vaccination to fight the ongoing Coronavirus disease 19 (COVID-19) pandemic. Molecules or extracts inhibiting the SARS-CoV-2 chymotripsin-like protease (3CL(Pro)) could contribute to reducing or suppressing SARS-CoV-2 replication. Using a targeted approach, we identified 17 plant products that are included in current and traditional cuisines as promising inhibitors of SARS-CoV-2 3CL(Pro) activity. Methanolic extracts were evaluated in vitro for inhibition of SARS-CoV-2 3CL(Pro) activity using a quenched fluorescence resonance energy transfer (FRET) assay. Extracts from turmeric (Curcuma longa) rhizomes, mustard (Brassica nigra) seeds, and wall rocket (Diplotaxis erucoides subsp. erucoides) at 500 microg mL(-1) displayed significant inhibition of the 3CL(Pro) activity, resulting in residual protease activities of 0.0%, 9.4%, and 14.9%, respectively. Using different extract concentrations, an IC(50) value of 15.74 microg mL(-1) was calculated for turmeric extract. Commercial curcumin inhibited the 3CL(Pro) activity, but did not fully account for the inhibitory effect of turmeric rhizomes extracts, suggesting that other components of the turmeric extract must also play a main role in inhibiting the 3CL(Pro) activity. Sinigrin, a major glucosinolate present in mustard seeds and wall rocket, did not have relevant 3CL(Pro) inhibitory activity; however, its hydrolysis product allyl isothiocyanate had an IC(50) value of 41.43 microg mL(-1). The current study identifies plant extracts and molecules that can be of interest in the search for treatments against COVID-19, acting as a basis for future chemical, in vivo, and clinical trials. ?Potential In Vitro Inhibition of Selected Plant Extracts against SARS-(epmc).pdf DeepDyve Pubget Overpricing