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10.3390/vaccines9060613 http://scihub22266oqcxt.onion/10.3390/vaccines9060613 34200441!8229131!34200441     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34200441&cmd=llinks): Failed to open stream: HTTP request failed! 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Optimization of Vero Cells Grown on a Polymer Fiber Carrier in a Disposable Bioreactor for Inactivated Coxsackievirus A16 Vaccine Development |pdf=|usr=}}{{34200441}} gab.com Text Optimization of Vero Cells Grown on a Polymer Fiber Carrier in a Disposable Bioreactor for Inactivated Coxsackievirus A16 Vaccine Development JO: Vaccines (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=34200441 #FOAvip At present, there are no vaccines available for hand, foot, and mouth disease, which is caused by Coxsackie virus A16 (CVA16) infection. In the present study, we isolated epidemic strains of CVA16 and optimized the production of the virus in Vero cells. The system comprised growing the infected cells on polymer fiber paper carriers in a serum-free medium containing 0.5% (w/v) lactalbumin hydrolysate a mini bioreactor. Disposable Bioflo310 and AmProtein Current perfusion bioreactors were used to monitor virus infection and Vero cell culture. The total number of cells increased from 1.5 x 10(9) to 3.0 x 10(10). In our optimized culture process, the virus titer reached 7.8 x 10(7) TCID(50)/mL at three days after infection. The inactivated CVA16 prepared from our optimized culture procedure elicited a slightly higher neutralizing antibody titer compared with that derived from routine culture procedures. These results will promote the large-scale production of inactivated CVA16 vaccines using nonwoven polymer fiber paper cell cultures. Twit Text FOAVip Optimization of Vero Cells Grown on a Polymer Fiber Carrier in a Disposable Bioreactor for Inactivated Coxsackievirus A16 Vaccine Development ????Vaccines (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=34200441 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34200441 #FOAvip English Wikipedia <ref>{{Cite pmid|34200441}}</ref> Optimization of Vero Cells Grown on a Polymer Fiber Carrier in a Disposable Bioreactor for Inactivated Coxsackievirus A16 Vaccine Development #MMPMID34200441 Chen K; Li C; Wang Y; Shen Z; Guo Y; Li X; Zhang Y Vaccines (Basel) 2021[Jun]; 9 (6): ä PMID34200441show ga At present, there are no vaccines available for hand, foot, and mouth disease, which is caused by Coxsackie virus A16 (CVA16) infection. In the present study, we isolated epidemic strains of CVA16 and optimized the production of the virus in Vero cells. The system comprised growing the infected cells on polymer fiber paper carriers in a serum-free medium containing 0.5% (w/v) lactalbumin hydrolysate a mini bioreactor. Disposable Bioflo310 and AmProtein Current perfusion bioreactors were used to monitor virus infection and Vero cell culture. The total number of cells increased from 1.5 x 10(9) to 3.0 x 10(10). In our optimized culture process, the virus titer reached 7.8 x 10(7) TCID(50)/mL at three days after infection. The inactivated CVA16 prepared from our optimized culture procedure elicited a slightly higher neutralizing antibody titer compared with that derived from routine culture procedures. These results will promote the large-scale production of inactivated CVA16 vaccines using nonwoven polymer fiber paper cell cultures. äOptimization of Vero Cells Grown on a Polymer Fiber Carrier in a Dispo(epmc).pdf DeepDyve Pubget Overpricing