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10.3390/ijms22116094 http://scihub22266oqcxt.onion/10.3390/ijms22116094 34198800!8201247!34198800     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Mol+Sci 2021 ; 22 (11): ä Nephropedia Template TP {{tp|p=34198800|t=2021. In Silico and In Vitro Analyses Validate Human MicroRNAs Targeting the SARS-CoV-2 3 -UTR |pdf=|usr=}}{{34198800}} gab.com Text In Silico and In Vitro Analyses Validate Human MicroRNAs Targeting the SARS-CoV-2 3 -UTR JO: Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=34198800 #FOAvip COVID-19 pandemic is caused by betacoronavirus SARS-CoV-2. The genome of this virus is composed of a single strand of RNA with 5' and 3'-UTR flanking a region of protein-coding ORFs closely resembling cells' mRNAs. MicroRNAs are endogenous post-transcriptional regulators that target mRNA to modulate protein expression and mediate cellular functions, including antiviral defense. In the present study, we carried out a bioinformatics screening to search for endogenous human microRNAs targeting the 3'-UTR of SARS-CoV-2. Results from the computational techniques allowed us to identify 10 potential candidates. The capacity of 3 of them, together with hsa-miR-138-5p, to target the SARS-CoV-2 3'-UTR was validated in vitro by gene reporter assays. Available information indicates that two of these microRNAs, namely, hsa-miR-3941 and hsa-miR-138-5p, combine effective targeting of SARS-CoV-2 genome with complementary antiviral or protective effects in the host cells that make them potential candidates for therapeutic treatment of most, if not all, COVID-19 variants known to date. All information obtained while conducting the present analysis is available at Open Science Framework repository. Twit Text FOAVip In Silico and In Vitro Analyses Validate Human MicroRNAs Targeting the SARS-CoV-2 3 -UTR ????Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=34198800 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34198800 #FOAvip English Wikipedia <ref>{{Cite pmid|34198800}}</ref> In Silico and In Vitro Analyses Validate Human MicroRNAs Targeting the SARS-CoV-2 3 -UTR #MMPMID34198800 Barreda-Manso MA; Nieto-Diaz M; Soto A; Munoz-Galdeano T; Reigada D; Maza RM Int J Mol Sci 2021[Jun]; 22 (11): ä PMID34198800show ga COVID-19 pandemic is caused by betacoronavirus SARS-CoV-2. The genome of this virus is composed of a single strand of RNA with 5' and 3'-UTR flanking a region of protein-coding ORFs closely resembling cells' mRNAs. MicroRNAs are endogenous post-transcriptional regulators that target mRNA to modulate protein expression and mediate cellular functions, including antiviral defense. In the present study, we carried out a bioinformatics screening to search for endogenous human microRNAs targeting the 3'-UTR of SARS-CoV-2. Results from the computational techniques allowed us to identify 10 potential candidates. The capacity of 3 of them, together with hsa-miR-138-5p, to target the SARS-CoV-2 3'-UTR was validated in vitro by gene reporter assays. Available information indicates that two of these microRNAs, namely, hsa-miR-3941 and hsa-miR-138-5p, combine effective targeting of SARS-CoV-2 genome with complementary antiviral or protective effects in the host cells that make them potential candidates for therapeutic treatment of most, if not all, COVID-19 variants known to date. All information obtained while conducting the present analysis is available at Open Science Framework repository. |3' Untranslated Regions[MESH] |Base Sequence[MESH] |Binding Sites[MESH] |COVID-19/genetics/pathology/virology[MESH] |Cell Line[MESH] |Genes, Reporter[MESH] |Genome, Viral[MESH] |Humans[MESH] |MicroRNAs/chemistry/*metabolism[MESH] |Open Reading Frames[MESH] |SARS-CoV-2/*genetics/isolation & purification[MESH]In Silico and In Vitro Analyses Validate Human MicroRNAs Targeting the(epmc).pdf DeepDyve Pubget Overpricing