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10.1042/BCJ20210199 http://scihub22266oqcxt.onion/10.1042/BCJ20210199 34198324!8286823!34198324     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biochem+J 2021 ; 478 (13): 2465-2479 Nephropedia Template TP {{tp|p=34198324|t=2021. Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of nsp15 endoribonuclease |pdf=|usr=}}{{34198324}} gab.com Text Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of nsp15 endoribonuclease JO: Biochem J http://www.kidney.de/pget.php?&tw=1&pmid=34198324 #FOAvip SARS-CoV-2 is responsible for COVID-19, a human disease that has caused over 2 million deaths, stretched health systems to near-breaking point and endangered economies of countries and families around the world. Antiviral treatments to combat COVID-19 are currently lacking. Remdesivir, the only antiviral drug approved for the treatment of COVID-19, can affect disease severity, but better treatments are needed. SARS-CoV-2 encodes 16 non-structural proteins (nsp) that possess different enzymatic activities with important roles in viral genome replication, transcription and host immune evasion. One key aspect of host immune evasion is performed by the uridine-directed endoribonuclease activity of nsp15. Here we describe the expression and purification of nsp15 recombinant protein. We have developed biochemical assays to follow its activity, and we have found evidence for allosteric behaviour. We screened a custom chemical library of over 5000 compounds to identify nsp15 endoribonuclease inhibitors, and we identified and validated NSC95397 as an inhibitor of nsp15 endoribonuclease in vitro. Although NSC95397 did not inhibit SARS-CoV-2 growth in VERO E6 cells, further studies will be required to determine the effect of nsp15 inhibition on host immune evasion. Twit Text FOAVip Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of nsp15 endoribonuclease ????Biochem J http://www.kidney.de/pget.php?&tw=1&pmid=34198324 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34198324 #FOAvip English Wikipedia <ref>{{Cite pmid|34198324}}</ref> Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of nsp15 endoribonuclease #MMPMID34198324 Canal B; Fujisawa R; McClure AW; Deegan TD; Wu M; Ulferts R; Weissmann F; Drury LS; Bertolin AP; Zeng J; Beale R; Howell M; Labib K; Diffley JFX Biochem J 2021[Jul]; 478 (13): 2465-2479 PMID34198324show ga SARS-CoV-2 is responsible for COVID-19, a human disease that has caused over 2 million deaths, stretched health systems to near-breaking point and endangered economies of countries and families around the world. Antiviral treatments to combat COVID-19 are currently lacking. Remdesivir, the only antiviral drug approved for the treatment of COVID-19, can affect disease severity, but better treatments are needed. SARS-CoV-2 encodes 16 non-structural proteins (nsp) that possess different enzymatic activities with important roles in viral genome replication, transcription and host immune evasion. One key aspect of host immune evasion is performed by the uridine-directed endoribonuclease activity of nsp15. Here we describe the expression and purification of nsp15 recombinant protein. We have developed biochemical assays to follow its activity, and we have found evidence for allosteric behaviour. We screened a custom chemical library of over 5000 compounds to identify nsp15 endoribonuclease inhibitors, and we identified and validated NSC95397 as an inhibitor of nsp15 endoribonuclease in vitro. Although NSC95397 did not inhibit SARS-CoV-2 growth in VERO E6 cells, further studies will be required to determine the effect of nsp15 inhibition on host immune evasion. |*Drug Evaluation, Preclinical[MESH] |Allosteric Regulation[MESH] |Animals[MESH] |Antiviral Agents/*chemistry/*pharmacology[MESH] |Chlorocebus aethiops[MESH] |Endoribonucleases/*antagonists & inhibitors/isolation & purification/metabolism[MESH] |Enzyme Assays[MESH] |Fluorescence[MESH] |High-Throughput Screening Assays[MESH] |In Vitro Techniques[MESH] |Kinetics[MESH] |Naphthoquinones/pharmacology[MESH] |Reproducibility of Results[MESH] |SARS-CoV-2/drug effects/*enzymology/growth & development[MESH] |Small Molecule Libraries/chemistry/*pharmacology[MESH] |Solutions[MESH] |Vero Cells[MESH]Identifying SARS-CoV-2 antiviral compounds by screening for small mole(epmc).pdf DeepDyve Pubget Overpricing