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10.1039/d1tb00428j http://scihub22266oqcxt.onion/10.1039/d1tb00428j 34196647!ä!34196647 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Mater+Chem+B 2021 ; 9 (28): 5636-5644 Nephropedia Template TP {{tp|p=34196647|t=2021. Selenium atom on phosphate enhances specificity and sensitivity of DNA polymerization and detection |pdf=|usr=}}{{34196647}} gab.com Text Selenium atom on phosphate enhances specificity and sensitivity of DNA polymerization and detection JO: J Mater Chem B http://www.kidney.de/pget.php?&tw=1&pmid=34196647 #FOAvip DNA polymerization is of high specificity in vivo. However, its specificity is much lower in vitro, which limits advanced applications of DNA polymerization in ultrasensitive nucleic acid detection. Herein, we report a unique mechanism of single selenium-atom modified dNTP (dNTPalphaSe) to enhance polymerization specificity. We have found that both dNTPalphaSe (approximately 660 fold) and Se-DNA (approximately 2.8 fold) have lower binding affinity to DNA polymerase than canonical ones, and the Se-DNA duplex has much lower melting-temperature (Tm) than the corresponding canonical DNA duplex. The reduced affinity and Tm can destabilize the substrate-primer-template-enzyme assembly, thereby largely slowing down the mismatch of DNA polymerization and enhancing the amplification specificity and in turn detection sensitivity. Furthermore, the Se-strategy enables us to develop the selenium enhanced specific isothermal amplification (SEA) for nucleic acid detection with high specificity and sensitivity (up to detection of single-digit copies), allowing convenient detection of clinical HPV and COVID-19 viruses in the low-copy number. Clearly, we have discovered the exciting mechanism for enhancing DNA polymerization accuracy, amplification specificity and detection sensitivity by SEA, up to two orders of magnitude higher. Twit Text FOAVip Selenium atom on phosphate enhances specificity and sensitivity of DNA polymerization and detection ????J Mater Chem B http://www.kidney.de/pget.php?&tw=1&pmid=34196647 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34196647 #FOAvip English Wikipedia <ref>{{Cite pmid|34196647}}</ref> Selenium atom on phosphate enhances specificity and sensitivity of DNA polymerization and detection #MMPMID34196647 Luo G; Zhang J; Yang M; He H; Huang Z J Mater Chem B 2021[Jul]; 9 (28): 5636-5644 PMID34196647show ga DNA polymerization is of high specificity in vivo. However, its specificity is much lower in vitro, which limits advanced applications of DNA polymerization in ultrasensitive nucleic acid detection. Herein, we report a unique mechanism of single selenium-atom modified dNTP (dNTPalphaSe) to enhance polymerization specificity. We have found that both dNTPalphaSe (approximately 660 fold) and Se-DNA (approximately 2.8 fold) have lower binding affinity to DNA polymerase than canonical ones, and the Se-DNA duplex has much lower melting-temperature (Tm) than the corresponding canonical DNA duplex. The reduced affinity and Tm can destabilize the substrate-primer-template-enzyme assembly, thereby largely slowing down the mismatch of DNA polymerization and enhancing the amplification specificity and in turn detection sensitivity. Furthermore, the Se-strategy enables us to develop the selenium enhanced specific isothermal amplification (SEA) for nucleic acid detection with high specificity and sensitivity (up to detection of single-digit copies), allowing convenient detection of clinical HPV and COVID-19 viruses in the low-copy number. Clearly, we have discovered the exciting mechanism for enhancing DNA polymerization accuracy, amplification specificity and detection sensitivity by SEA, up to two orders of magnitude higher. |DNA, Viral/*analysis/biosynthesis[MESH] |Human papillomavirus 16/*isolation & purification/metabolism[MESH] |Humans[MESH] |Nucleic Acid Amplification Techniques[MESH] |Phosphates/*chemistry[MESH] |Polymerization[MESH] |SARS-CoV-2/*isolation & purification/metabolism[MESH] |Selenium/*chemistry[MESH]Selenium atom on phosphate enhances specificity and sensitivity of DNA(epmc).pdf DeepDyve Pubget Overpricing