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10.1016/j.xpro.2021.100499 http://scihub22266oqcxt.onion/10.1016/j.xpro.2021.100499 34195671!8233161!34195671     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       STAR+Protoc 2021 ; 2 (3): 100499 Nephropedia Template TP {{tp|p=34195671|t=2021. Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice |pdf=|usr=}}{{34195671}} gab.com Text Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice JO: STAR Protoc http://www.kidney.de/pget.php?&tw=1&pmid=34195671 #FOAvip Location of immune cells that form the germinal center reaction within secondary lymphoid tissues can be characterized using confocal microscopy. Here, we present an optimized immunofluorescence staining protocol to image germinal center structures in fixed/frozen spleen sections from ChAdOx1 nCoV-19 immunized mice. This protocol can be adapted to identify other cell types within secondary lymphoid tissues. For complete information on the generation and use of this protocol to examine immune responses to the COVID vaccine ChAdOx1 nCoV-19, please refer to Silva-Cayetano et al. (2020). Twit Text FOAVip Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice ????STAR Protoc http://www.kidney.de/pget.php?&tw=1&pmid=34195671 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34195671 #FOAvip English Wikipedia <ref>{{Cite pmid|34195671}}</ref> Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice #MMPMID34195671 Fra-Bido S; Walker SA; Innocentin S; Linterman MA STAR Protoc 2021[Sep]; 2 (3): 100499 PMID34195671show ga Location of immune cells that form the germinal center reaction within secondary lymphoid tissues can be characterized using confocal microscopy. Here, we present an optimized immunofluorescence staining protocol to image germinal center structures in fixed/frozen spleen sections from ChAdOx1 nCoV-19 immunized mice. This protocol can be adapted to identify other cell types within secondary lymphoid tissues. For complete information on the generation and use of this protocol to examine immune responses to the COVID vaccine ChAdOx1 nCoV-19, please refer to Silva-Cayetano et al. (2020). |Animals[MESH] |COVID-19/diagnostic imaging/immunology/*prevention & control/virology[MESH] |ChAdOx1 nCoV-19/*administration & dosage[MESH] |Fluorescent Antibody Technique/methods/*standards[MESH] |Germinal Center/*drug effects/immunology/pathology/virology[MESH] |Immunization, Secondary/*methods[MESH] |Immunogenicity, Vaccine[MESH] |Male[MESH] |Mice[MESH] |SARS-CoV-2/*immunology[MESH]Optimized immunofluorescence staining protocol for imaging germinal ce(epmc).pdf DeepDyve Pubget Overpricing