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10.1186/s12879-021-06347-6

http://scihub22266oqcxt.onion/10.1186/s12879-021-06347-6
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suck abstract from ncbi


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pmid34193072      BMC+Infect+Dis 2021 ; 21 (1): 623
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  • Comparative evaluation of the Thermo fisher TaqPath COVID-19 combo kit with the Cepheid Xpert(R) Xpress SARS-CoV-2 assay for detecting SARS-CoV-2 in nasopharyngeal specimens #MMPMID34193072
  • Granato PA; Kimball SR; Alkins BR; Cross DC; Unz MM
  • BMC Infect Dis 2021[Jun]; 21 (1): 623 PMID34193072show ga
  • PURPOSE: With over 50 SARS-CoV-2 gene amplification assays that have been EUA cleared with minimal experimental validation performed, it is likely that not all of these assays are comparable in their ability to detect SARS-CoV-2 in clinical specimens. Thermo Fisher Scientific is a relatively new company in the molecular diagnostics field and the purpose of this study was to compare the performance of the Thermo Fisher TaqPath Combo Kit with an established test, the Cepheid Xpert(R) Xpress SARS-CoV-2 assay, for its ability to detect SARS-CoV-2 in nasopharyngeal specimens. METHODS: A total of 300 randomly selected nasopharyngeal specimens were evaluated and tested by the TaqPath and GeneXpert assays. Discordant test specimens were arbitrated by performing an alternative PCR assay and Sanger sequencing. RESULTS: The TaqPath assay had a 96.7 and 99.6% positive and negative agreement respectively when compared to the Xpert Xpress test. However, after test arbitration, the three discordant specimens were arbitrated in favor of the TaqPath assay producing a positive and negative percent agreement of 100% for the TaqPath Combo Kit while the Xpress SARS-CoV-2 assay had a positive and negative percent agreement of 98.3 and 99.2% respectively. CONCLUSIONS: The TaqPath Combo Kit is a high complexity assay that compares favorably with the Xpert Xpress test and can be reliably used for the detection of SARS-CoV-2 in nasopharyngeal specimens.
  • |*Molecular Diagnostic Techniques[MESH]
  • |*SARS-CoV-2[MESH]
  • |COVID-19 Nucleic Acid Testing/*methods[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |Humans[MESH]
  • |Nasopharynx/virology[MESH]


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