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10.1021/acs.analchem.1c00886

http://scihub22266oqcxt.onion/10.1021/acs.analchem.1c00886
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34192453!ä!34192453

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suck abstract from ncbi


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pmid34192453      Anal+Chem 2021 ; 93 (28): 9719-9727
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  • SARS-CoV-2 Detection with De Novo-Designed Synthetic Riboregulators #MMPMID34192453
  • Koksaldi IC; Kose S; Ahan RE; Haciosmanoglu N; Sahin Kehribar E; Gungen MA; Bastug A; Dinc B; Bodur H; Ozkul A; Seker UOS
  • Anal Chem 2021[Jul]; 93 (28): 9719-9727 PMID34192453show ga
  • SARS-CoV-2 is a human pathogen and the main cause of COVID-19 disease, announced as a global pandemic by the World Health Organization. COVID-19 is characterized by severe conditions, and early diagnosis can make dramatic changes for both personal and public health. Low-cost, easy-to-use diagnostic capabilities can have a very critical role in controlling the transmission of the disease. Here, we are reporting a state-of-the-art diagnostic tool developed with an in vitro synthetic biology approach by employing engineered de novo riboregulators. Our design coupled with a home-made point-of-care device can detect and report the presence of SARS-CoV-2-specific genes. The presence of SARS-CoV-2-related genes triggers the translation of sfGFP mRNAs, resulting in a green fluorescence output. The approach proposed here has the potential of being a game changer in SARS-CoV-2 diagnostics by providing an easy-to-run, low-cost diagnostic capability.
  • |*COVID-19[MESH]
  • |*SARS-CoV-2[MESH]
  • |Humans[MESH]
  • |Pandemics[MESH]


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