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10.17843/rpmesp.2021.381.7154 http://scihub22266oqcxt.onion/10.17843/rpmesp.2021.381.7154 34190927!ä!34190927 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Rev+Peru+Med+Exp+Salud+Publica 2021 ; 38 (1): 7-16 Nephropedia Template TP {{tp|p=34190927|t=2021. Estandarizacion y validacion de una prueba molecular RT-LAMP in house para el diagnostico de SARS-CoV-2 |pdf=|usr=}}{{34190927}} gab.com Text Estandarizacion y validacion de una prueba molecular RT-LAMP in house para el diagnostico de SARS-CoV-2 JO: Rev Peru Med Exp Salud Publica http://www.kidney.de/pget.php?&tw=1&pmid=34190927 #FOAvip OBJECTIVES: To standardize and validate an in-house RT-LAMP test for the detection of SARS-CoV-2, based on laboratory and field assays using samples from COVID-19 suspected patients. MATERIALS AND METHODS: An in-house SARS-CoV-2 RT-LAMP molecular test was standardized, establishing the detection limit with Vero cells of isolated Peruvian strains of SARS-CoV-2, and the robustness to various concentrations of primers. The laboratory validation was performed with 384 nasal and pharyngeal swab samples (UFH) obtained between March and July 2020. The field validation was performed with 383 UFH obtained from COVID-19 suspected symptomatic cases. All samples were tested by RT-LAMP and RT-qPCR. The RT-qPCR was considered as the reference standard test. The concordance measures and diagnostic performance were calculated. RESULTS: The detection limit was consistent in cases with Ct <30 in both tests, showing efficiency to detect up to 1000 copies/muL of the target gene. Robustness was evidenced with half of the primer concentrations and 20 muL of final volume. Absence of amplification was identified for other HCoVs. Concordance showed a kappa index of 0.88 (95% CI: 0.83-0.93) and 0.89 (95% CI: 0.84 - 0.94) in laboratory and field settings, respectively. The sensitivity value in the laboratory was 87.4% (95% CI: 80.8 - 92.4) and 88.1% in the field (95% CI: 81.6 - 92.9). The specificity value in both settings was 98.8% (95% CI: 96.4-99.7). CONCLUSIONS: The in-house SARS-CoV-2 RT-LAMP test was successfully validated based on its adequate robustness, no cross-reactions, good concordance, and diagnostic performance compared to RT-qPCR. Twit Text FOAVip Estandarizacion y validacion de una prueba molecular RT-LAMP in house para el diagnostico de SARS-CoV-2 ????Rev Peru Med Exp Salud Publica http://www.kidney.de/pget.php?&tw=1&pmid=34190927 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34190927 #FOAvip English Wikipedia <ref>{{Cite pmid|34190927}}</ref> Estandarizacion y validacion de una prueba molecular RT-LAMP in house para el diagnostico de SARS-CoV-2 #MMPMID34190927 Escalante-Maldonado O; Vidal-Anzardo M; Donaires F; Solis-Sanchez G; Gallesi I; Pampa-Espinoza L; Huaringa M; Rojas-Serrano N; Garcia C; Angles-Yanqui E; Gavilan RG; Duraes-Carvalho R; Mendez-Rico J; Cabezas C; Marques-Simas PV Rev Peru Med Exp Salud Publica 2021[Jan]; 38 (1): 7-16 PMID34190927show ga OBJECTIVES: To standardize and validate an in-house RT-LAMP test for the detection of SARS-CoV-2, based on laboratory and field assays using samples from COVID-19 suspected patients. MATERIALS AND METHODS: An in-house SARS-CoV-2 RT-LAMP molecular test was standardized, establishing the detection limit with Vero cells of isolated Peruvian strains of SARS-CoV-2, and the robustness to various concentrations of primers. The laboratory validation was performed with 384 nasal and pharyngeal swab samples (UFH) obtained between March and July 2020. The field validation was performed with 383 UFH obtained from COVID-19 suspected symptomatic cases. All samples were tested by RT-LAMP and RT-qPCR. The RT-qPCR was considered as the reference standard test. The concordance measures and diagnostic performance were calculated. RESULTS: The detection limit was consistent in cases with Ct <30 in both tests, showing efficiency to detect up to 1000 copies/muL of the target gene. Robustness was evidenced with half of the primer concentrations and 20 muL of final volume. Absence of amplification was identified for other HCoVs. Concordance showed a kappa index of 0.88 (95% CI: 0.83-0.93) and 0.89 (95% CI: 0.84 - 0.94) in laboratory and field settings, respectively. The sensitivity value in the laboratory was 87.4% (95% CI: 80.8 - 92.4) and 88.1% in the field (95% CI: 81.6 - 92.9). The specificity value in both settings was 98.8% (95% CI: 96.4-99.7). CONCLUSIONS: The in-house SARS-CoV-2 RT-LAMP test was successfully validated based on its adequate robustness, no cross-reactions, good concordance, and diagnostic performance compared to RT-qPCR. |*COVID-19[MESH] |*SARS-CoV-2[MESH] |Animals[MESH] |Chlorocebus aethiops[MESH] |Humans[MESH] |Molecular Diagnostic Techniques[MESH] |Nucleic Acid Amplification Techniques[MESH] |RNA, Viral[MESH] |Reference Standards[MESH] |Sensitivity and Specificity[MESH]Estandarizacion y validacion de una prueba molecular RT-LAMP in house (epmc).pdf DeepDyve Pubget Overpricing