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10.1038/s41587-021-00966-9 http://scihub22266oqcxt.onion/10.1038/s41587-021-00966-9 34188222!8678193!34188222     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Biotechnol 2021 ; 39 (12): 1556-1562 Nephropedia Template TP {{tp|p=34188222|t=2021. LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding |pdf=|usr=}}{{34188222}} gab.com Text LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding JO: Nat Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=34188222 #FOAvip Frequent testing of large population groups combined with contact tracing and isolation measures will be crucial for containing Coronavirus Disease 2019 outbreaks. Here we present LAMP-Seq, a modified, highly scalable reverse transcription loop-mediated isothermal amplification (RT-LAMP) method. Unpurified biosamples are barcoded and amplified in a single heat step, and pooled products are analyzed en masse by sequencing. Using commercial reagents, LAMP-Seq has a limit of detection of ~2.2 molecules per microl at 95% confidence and near-perfect specificity for severe acute respiratory syndrome coronavirus 2 given its sequence readout. Clinical validation of an open-source protocol with 676 swab samples, 98 of which were deemed positive by standard RT-qPCR, demonstrated 100% sensitivity in individuals with cycle threshold values of up to 33 and a specificity of 99.7%, at a very low material cost. With a time-to-result of fewer than 24 h, low cost and little new infrastructure requirement, LAMP-Seq can be readily deployed for frequent testing as part of an integrated public health surveillance program. Twit Text FOAVip LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding ????Nat Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=34188222 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34188222 #FOAvip English Wikipedia <ref>{{Cite pmid|34188222}}</ref> LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using molecular barcoding #MMPMID34188222 Ludwig KU; Schmithausen RM; Li D; Jacobs ML; Hollstein R; Blumenstock K; Liebing J; Slabicki M; Ben-Shmuel A; Israeli O; Weiss S; Ebert TS; Paran N; Rudiger W; Wilbring G; Feldman D; Lippke B; Ishorst N; Hochfeld LM; Beins EC; Kaltheuner IH; Schmitz M; Wohler A; Dohla M; Sib E; Jentzsch M; Moench EC; Borrajo JD; Strecker J; Reinhardt J; Cleary B; Geyer M; Holzel M; Macrae R; Nothen MM; Hoffmann P; Exner M; Regev A; Zhang F; Schmid-Burgk JL Nat Biotechnol 2021[Dec]; 39 (12): 1556-1562 PMID34188222show ga Frequent testing of large population groups combined with contact tracing and isolation measures will be crucial for containing Coronavirus Disease 2019 outbreaks. Here we present LAMP-Seq, a modified, highly scalable reverse transcription loop-mediated isothermal amplification (RT-LAMP) method. Unpurified biosamples are barcoded and amplified in a single heat step, and pooled products are analyzed en masse by sequencing. Using commercial reagents, LAMP-Seq has a limit of detection of ~2.2 molecules per microl at 95% confidence and near-perfect specificity for severe acute respiratory syndrome coronavirus 2 given its sequence readout. Clinical validation of an open-source protocol with 676 swab samples, 98 of which were deemed positive by standard RT-qPCR, demonstrated 100% sensitivity in individuals with cycle threshold values of up to 33 and a specificity of 99.7%, at a very low material cost. With a time-to-result of fewer than 24 h, low cost and little new infrastructure requirement, LAMP-Seq can be readily deployed for frequent testing as part of an integrated public health surveillance program. |*COVID-19/diagnosis[MESH] |COVID-19 Testing/*methods[MESH] |Humans[MESH] |Molecular Diagnostic Techniques/*methods[MESH]LAMP-Seq enables sensitive, multiplexed COVID-19 diagnostics using mol(epmc).pdf DeepDyve Pubget Overpricing