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10.1038/s41598-021-92677-z http://scihub22266oqcxt.onion/10.1038/s41598-021-92677-z 34183720!8238998!34183720     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34183720&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2021 ; 11 (1): 13378 Nephropedia Template TP {{tp|p=34183720|t=2021. A rapid near-patient detection system for SARS-CoV-2 using saliva |pdf=|usr=}}{{34183720}} gab.com Text A rapid near-patient detection system for SARS-CoV-2 using saliva JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34183720 #FOAvip The highly infectious nature of SARS-CoV-2 necessitates the use of widespread testing to control the spread of the virus. Presently, the standard molecular testing method (reverse transcriptase-polymerase chain reaction, RT-PCR) is restricted to the laboratory, time-consuming, and costly. This increases the turnaround time for getting test results. This study sought to develop a rapid, near-patient saliva-based test for COVID-19 (Saliva-Dry LAMP) with similar accuracy to that of standard RT-PCR tests. A lyophilized dual-target reverse transcription-loop-mediated isothermal amplification (RT-LAMP) test with fluorometric detection by the naked eye was developed. The assay relies on dry reagents that are room temperature stable. A device containing a centrifuge, heat block, and blue LED light system was manufactured to reduce the cost of performing the assay. This test has a limit of detection of 1 copy/microL and achieved a positive percent agreement of 100% [95% CI 88.43% to 100.0%] and a negative percent agreement of 96.7% [95% CI 82.78-99.92%] relative to a reference standard test. Saliva-Dry LAMP can be completed in 105 min. Precision, cross-reactivity, and interfering substances analysis met international regulatory standards. The combination of ease of sample collection, dry reagents, visual detection, low capital equipment cost, and excellent analytical sensitivity make Saliva-Dry LAMP particularly useful for resource-limited settings. Twit Text FOAVip A rapid near-patient detection system for SARS-CoV-2 using saliva ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34183720 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34183720 #FOAvip English Wikipedia <ref>{{Cite pmid|34183720}}</ref> A rapid near-patient detection system for SARS-CoV-2 using saliva #MMPMID34183720 Toppings NB; Mohon AN; Lee Y; Kumar H; Lee D; Kapoor R; Singh G; Oberding L; Abdullah O; Kim K; Berenger BM; Pillai DR Sci Rep 2021[Jun]; 11 (1): 13378 PMID34183720show ga The highly infectious nature of SARS-CoV-2 necessitates the use of widespread testing to control the spread of the virus. Presently, the standard molecular testing method (reverse transcriptase-polymerase chain reaction, RT-PCR) is restricted to the laboratory, time-consuming, and costly. This increases the turnaround time for getting test results. This study sought to develop a rapid, near-patient saliva-based test for COVID-19 (Saliva-Dry LAMP) with similar accuracy to that of standard RT-PCR tests. A lyophilized dual-target reverse transcription-loop-mediated isothermal amplification (RT-LAMP) test with fluorometric detection by the naked eye was developed. The assay relies on dry reagents that are room temperature stable. A device containing a centrifuge, heat block, and blue LED light system was manufactured to reduce the cost of performing the assay. This test has a limit of detection of 1 copy/microL and achieved a positive percent agreement of 100% [95% CI 88.43% to 100.0%] and a negative percent agreement of 96.7% [95% CI 82.78-99.92%] relative to a reference standard test. Saliva-Dry LAMP can be completed in 105 min. Precision, cross-reactivity, and interfering substances analysis met international regulatory standards. The combination of ease of sample collection, dry reagents, visual detection, low capital equipment cost, and excellent analytical sensitivity make Saliva-Dry LAMP particularly useful for resource-limited settings. |COVID-19/*diagnosis/virology[MESH] |Fluorometry[MESH] |Humans[MESH] |Limit of Detection[MESH] |Molecular Diagnostic Techniques/instrumentation/*methods/standards[MESH] |Nucleic Acid Amplification Techniques/instrumentation/*methods/standards[MESH] |RNA, Viral/*analysis/standards[MESH] |Reference Standards[MESH] |SARS-CoV-2/genetics/isolation & purification[MESH] |Saliva/*virology[MESH] |Sensitivity and Specificity[MESH]A rapid near-patient detection system for SARS-CoV-2 using saliva (epmc).pdf DeepDyve Pubget Overpricing