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10.1016/j.jcv.2021.104903 http://scihub22266oqcxt.onion/10.1016/j.jcv.2021.104903 34182300!8213512!34182300     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Virol 2021 ; 141 (ä): 104903 Nephropedia Template TP {{tp|p=34182300|t=2021. Rapid screening method for the detection of SARS-CoV-2 variants of concern |pdf=|usr=}}{{34182300}} gab.com Text Rapid screening method for the detection of SARS-CoV-2 variants of concern JO: J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=34182300 #FOAvip BACKGROUND: Comprehensive and up-to-date monitoring of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VOC) is crucial as these are characterized by their increased transmissibility, immune evasion and virulence. OBJECTIVES: To describe the wide-scale implementation of a reverse transcriptase polymerase chain reaction (RT-PCR) multiple variants assay with melting curve analysis as a routine procedure. STUDY DESIGN: We prospectively performed multiple variants RT-PCR on consecutive SARS-CoV-2 RT-PCR positive samples from patients, healthcare workers and nursing home residents from our hospital catchment area. This technique was implemented in our automated Roche FLOW system with a turn-around time of 6 h. RESULTS: Between February 1 and May 2, 2021, 989 samples were tested by the variant RT-PCR. Our method was validated by comparison of variant RT-PCR to whole genome sequencing testing. We observed an increase over time in the proportion of UK variant that became the dominant variant, and the concurrent emergence of the South-African and Brazilian variants. Prompt public health responses for infection control were possible because of this rapid screening method, resulting in early detection and reduction of unnoticed spread of VOC as early as possible. CONCLUSION: A variant RT-PCR with additional melting curve analyses is a feasible, rapid and efficient screening strategy that can be implemented in routine microbiological laboratories. Twit Text FOAVip Rapid screening method for the detection of SARS-CoV-2 variants of concern ????J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=34182300 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34182300 #FOAvip English Wikipedia <ref>{{Cite pmid|34182300}}</ref> Rapid screening method for the detection of SARS-CoV-2 variants of concern #MMPMID34182300 Ong DSY; Koeleman JGM; Vaessen N; Breijer S; Paltansing S; de Man P J Clin Virol 2021[Aug]; 141 (ä): 104903 PMID34182300show ga BACKGROUND: Comprehensive and up-to-date monitoring of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VOC) is crucial as these are characterized by their increased transmissibility, immune evasion and virulence. OBJECTIVES: To describe the wide-scale implementation of a reverse transcriptase polymerase chain reaction (RT-PCR) multiple variants assay with melting curve analysis as a routine procedure. STUDY DESIGN: We prospectively performed multiple variants RT-PCR on consecutive SARS-CoV-2 RT-PCR positive samples from patients, healthcare workers and nursing home residents from our hospital catchment area. This technique was implemented in our automated Roche FLOW system with a turn-around time of 6 h. RESULTS: Between February 1 and May 2, 2021, 989 samples were tested by the variant RT-PCR. Our method was validated by comparison of variant RT-PCR to whole genome sequencing testing. We observed an increase over time in the proportion of UK variant that became the dominant variant, and the concurrent emergence of the South-African and Brazilian variants. Prompt public health responses for infection control were possible because of this rapid screening method, resulting in early detection and reduction of unnoticed spread of VOC as early as possible. CONCLUSION: A variant RT-PCR with additional melting curve analyses is a feasible, rapid and efficient screening strategy that can be implemented in routine microbiological laboratories. |*COVID-19[MESH] |*SARS-CoV-2[MESH] |Health Personnel[MESH] |Humans[MESH]Rapid screening method for the detection of SARS-CoV-2 variants of con(epmc).pdf DeepDyve Pubget Overpricing