Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1152/ajpendo.00174.2021 http://scihub22266oqcxt.onion/10.1152/ajpendo.00174.2021 34181461!8328521!34181461     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Am+J+Physiol+Endocrinol+Metab 2021 ; 321 (2): E246-E251 Nephropedia Template TP {{tp|p=34181461|t=2021. Vitamin D and lumisterol novel metabolites can inhibit SARS-CoV-2 replication machinery enzymes |pdf=|usr=}}{{34181461}} gab.com Text Vitamin D and lumisterol novel metabolites can inhibit SARS-CoV-2 replication machinery enzymes JO: Am J Physiol Endocrinol Metab http://www.kidney.de/pget.php?&tw=1&pmid=34181461 #FOAvip Vitamin D deficiency significantly correlates with the severity of SARS-CoV-2 infection. Molecular docking-based virtual screening studies predict that novel vitamin D and related lumisterol hydroxymetabolites are able to bind to the active sites of two SARS-CoV-2 transcription machinery enzymes with high affinity. These enzymes are the main protease (M(pro)) and RNA-dependent RNA polymerase (RdRP), which play important roles in viral replication and establishing infection. Based on predicted binding affinities and specific interactions, we identified 10 vitamin D3 (D3) and lumisterol (L3) analogs as likely binding partners of SARS-CoV-2 M(pro) and RdRP and, therefore, tested their ability to inhibit these enzymes. Activity measurements demonstrated that 25(OH)L3, 24(OH)L3, and 20(OH)7DHC are the most effective of the hydroxymetabolites tested at inhibiting the activity of SARS-CoV-2 M(pro) causing 10%-19% inhibition. These same derivatives as well as other hydroxylumisterols and hydroxyvitamin D3 metabolites inhibited RdRP by 50%-60%. Thus, inhibition of these enzymes by vitamin D and lumisterol metabolites may provide a novel approach to hindering the SARS-CoV-2 infection.NEW & NOTEWORTHY Active forms of vitamin D and lumisterol can inhibit SARS-CoV-2 replication machinery enzymes, which indicates that novel vitamin D and lumisterol metabolites are candidates for antiviral drug research. Twit Text FOAVip Vitamin D and lumisterol novel metabolites can inhibit SARS-CoV-2 replication machinery enzymes ????Am J Physiol Endocrinol Metab http://www.kidney.de/pget.php?&tw=1&pmid=34181461 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34181461 #FOAvip English Wikipedia <ref>{{Cite pmid|34181461}}</ref> Vitamin D and lumisterol novel metabolites can inhibit SARS-CoV-2 replication machinery enzymes #MMPMID34181461 Qayyum S; Mohammad T; Slominski RM; Hassan MI; Tuckey RC; Raman C; Slominski AT Am J Physiol Endocrinol Metab 2021[Aug]; 321 (2): E246-E251 PMID34181461show ga Vitamin D deficiency significantly correlates with the severity of SARS-CoV-2 infection. Molecular docking-based virtual screening studies predict that novel vitamin D and related lumisterol hydroxymetabolites are able to bind to the active sites of two SARS-CoV-2 transcription machinery enzymes with high affinity. These enzymes are the main protease (M(pro)) and RNA-dependent RNA polymerase (RdRP), which play important roles in viral replication and establishing infection. Based on predicted binding affinities and specific interactions, we identified 10 vitamin D3 (D3) and lumisterol (L3) analogs as likely binding partners of SARS-CoV-2 M(pro) and RdRP and, therefore, tested their ability to inhibit these enzymes. Activity measurements demonstrated that 25(OH)L3, 24(OH)L3, and 20(OH)7DHC are the most effective of the hydroxymetabolites tested at inhibiting the activity of SARS-CoV-2 M(pro) causing 10%-19% inhibition. These same derivatives as well as other hydroxylumisterols and hydroxyvitamin D3 metabolites inhibited RdRP by 50%-60%. Thus, inhibition of these enzymes by vitamin D and lumisterol metabolites may provide a novel approach to hindering the SARS-CoV-2 infection.NEW & NOTEWORTHY Active forms of vitamin D and lumisterol can inhibit SARS-CoV-2 replication machinery enzymes, which indicates that novel vitamin D and lumisterol metabolites are candidates for antiviral drug research. |Antiviral Agents/chemistry/*pharmacology[MESH] |Ergosterol/analogs & derivatives/chemistry/*metabolism/pharmacology[MESH] |Molecular Docking Simulation[MESH] |RNA-Dependent RNA Polymerase/*antagonists & inhibitors/chemistry[MESH] |SARS-CoV-2/*drug effects/physiology[MESH] |Virus Replication/*drug effects[MESH]Vitamin D and lumisterol novel metabolites can inhibit SARS-CoV-2 repl(epmc).pdf DeepDyve Pubget Overpricing