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10.1002/jcla.23835 http://scihub22266oqcxt.onion/10.1002/jcla.23835 34181279!8274989!34181279     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34181279&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Lab+Anal 2021 ; 35 (7): e23835 Nephropedia Template TP {{tp|p=34181279|t=2021. Pooled testing for SARS-CoV-2 infection in an automated high-throughput platform |pdf=|usr=}}{{34181279}} gab.com Text Pooled testing for SARS-CoV-2 infection in an automated high-throughput platform JO: J Clin Lab Anal http://www.kidney.de/pget.php?&tw=1&pmid=34181279 #FOAvip BACKGROUND: Active detection of SARS-CoV-2 infection through testing is elementary for the control of COVID-19 pandemic. The implementation of large-scale RT-PCR testing has led to a rise in the demand for testing kits whose availability is always a concern. OBJECTIVE: To find out the feasibility of pooled testing in a high-throughput platform. METHODOLOGY: Pooled testing was conducted in Roche cobas 6800 in 2 methods. Firstly, the simple two-stage testing algorithm was conducted for 1410 samples individually and then as pooled samples. Secondly, we evaluated the sensitivity of cobas 6800 for the detection of a single positive sample within a pool of negative samples. RESULTS: Implementing the five-sample Dorfman pooling to test 1410 samples, we identified 42 (2.9%) individual SARS-CoV-2-positive samples and 27 (9.5%) positive pool samples. The pooling strategy precisely identified all the positive samples. All individually negative samples were also accurately determined by pooling. There was 100% sensitivity of detecting positive samples in a pool of negative samples even up to 1:64 dilution. There was a threefold increase in total throughput in one-third of the cost per day. CONCLUSION: A high-throughput platform such as Cobas 6800 can effectively increase the testing capacity by twofold to threefold by adopting the pooled testing strategy for successful management of SARS-CoV-2 and helping in the containment of community transmission. Twit Text FOAVip Pooled testing for SARS-CoV-2 infection in an automated high-throughput platform ????J Clin Lab Anal http://www.kidney.de/pget.php?&tw=1&pmid=34181279 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34181279 #FOAvip English Wikipedia <ref>{{Cite pmid|34181279}}</ref> Pooled testing for SARS-CoV-2 infection in an automated high-throughput platform #MMPMID34181279 Dash GC; Rout UK; Nanda RR; Parai D; Choudhary HR; Kanungo S; Palo SK; Kshatri JS; Turuk J; Mishra BK; Pati S; Bhattacharya D J Clin Lab Anal 2021[Jul]; 35 (7): e23835 PMID34181279show ga BACKGROUND: Active detection of SARS-CoV-2 infection through testing is elementary for the control of COVID-19 pandemic. The implementation of large-scale RT-PCR testing has led to a rise in the demand for testing kits whose availability is always a concern. OBJECTIVE: To find out the feasibility of pooled testing in a high-throughput platform. METHODOLOGY: Pooled testing was conducted in Roche cobas 6800 in 2 methods. Firstly, the simple two-stage testing algorithm was conducted for 1410 samples individually and then as pooled samples. Secondly, we evaluated the sensitivity of cobas 6800 for the detection of a single positive sample within a pool of negative samples. RESULTS: Implementing the five-sample Dorfman pooling to test 1410 samples, we identified 42 (2.9%) individual SARS-CoV-2-positive samples and 27 (9.5%) positive pool samples. The pooling strategy precisely identified all the positive samples. All individually negative samples were also accurately determined by pooling. There was 100% sensitivity of detecting positive samples in a pool of negative samples even up to 1:64 dilution. There was a threefold increase in total throughput in one-third of the cost per day. CONCLUSION: A high-throughput platform such as Cobas 6800 can effectively increase the testing capacity by twofold to threefold by adopting the pooled testing strategy for successful management of SARS-CoV-2 and helping in the containment of community transmission. |Adolescent[MESH] |Adult[MESH] |Aged[MESH] |COVID-19 Testing/*methods[MESH] |COVID-19/*diagnosis/*virology[MESH] |Child[MESH] |Female[MESH] |High-Throughput Screening Assays/*methods[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |SARS-CoV-2/*physiology[MESH]Pooled testing for SARS-CoV-2 infection in an automated high-throughpu(epmc).pdf DeepDyve Pubget Overpricing