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10.1016/j.xpro.2021.100671 http://scihub22266oqcxt.onion/10.1016/j.xpro.2021.100671 34179835!8214198!34179835     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34179835&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       STAR+Protoc 2021 ; 2 (3): 100671 Nephropedia Template TP {{tp|p=34179835|t=2021. A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients |pdf=|usr=}}{{34179835}} gab.com Text A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients JO: STAR Protoc http://www.kidney.de/pget.php?&tw=1&pmid=34179835 #FOAvip One of the key public health strategies in coronavirus 2019 disease (COVID-19) management is the early detection of infected individuals to limit the transmission. As a result, serological assays have been developed to complement PCR-based assays. Here, we report the development of a flow cytometry-based assay to detect antibodies against full-length SARS-CoV-2 spike protein (S protein) in patients with COVID-19. The assay is time-efficient and sensitive, being able to capture the wider repertoire of antibodies against the S protein. For complete details on the use and execution of this protocol, please refer to Goh et al. (2021). Twit Text FOAVip A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients ????STAR Protoc http://www.kidney.de/pget.php?&tw=1&pmid=34179835 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34179835 #FOAvip English Wikipedia <ref>{{Cite pmid|34179835}}</ref> A flow cytometry-based assay for serological detection of anti-spike antibodies in COVID-19 patients #MMPMID34179835 Goh YS; Ng LFP; Renia L STAR Protoc 2021[Sep]; 2 (3): 100671 PMID34179835show ga One of the key public health strategies in coronavirus 2019 disease (COVID-19) management is the early detection of infected individuals to limit the transmission. As a result, serological assays have been developed to complement PCR-based assays. Here, we report the development of a flow cytometry-based assay to detect antibodies against full-length SARS-CoV-2 spike protein (S protein) in patients with COVID-19. The assay is time-efficient and sensitive, being able to capture the wider repertoire of antibodies against the S protein. For complete details on the use and execution of this protocol, please refer to Goh et al. (2021). |Antibodies, Neutralizing/*blood/immunology[MESH] |COVID-19 Serological Testing/*methods[MESH] |COVID-19/blood/*diagnosis/immunology/virology[MESH] |Enzyme-Linked Immunosorbent Assay/*methods[MESH] |Flow Cytometry/*methods[MESH] |Humans[MESH] |SARS-CoV-2/*immunology[MESH]A flow cytometry-based assay for serological detection of anti-spike a(epmc).pdf DeepDyve Pubget Overpricing