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10.1016/j.jmii.2021.03.007 http://scihub22266oqcxt.onion/10.1016/j.jmii.2021.03.007 34176764!7997684!34176764     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34176764&cmd=llinks): Failed to open stream: HTTP request failed! 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Gene signatures and potential therapeutic targets of Middle East respiratory syndrome coronavirus (MERS-CoV)-infected human lung adenocarcinoma epithelial cells |pdf=|usr=}}{{34176764}} gab.com Text Gene signatures and potential therapeutic targets of Middle East respiratory syndrome coronavirus (MERS-CoV)-infected human lung adenocarcinoma epithelial cells JO: J Microbiol Immunol Infect http://www.kidney.de/pget.php?&tw=1&pmid=34176764 #FOAvip BACKGROUND: Pathogenic coronaviruses include Middle East respiratory syndrome coronavirus (MERS-CoV), severe acute respiratory syndrome coronavirus (SARS-CoV), and SARS-CoV-2. These viruses have induced outbreaks worldwide, and there are currently no effective medications against them. Therefore, there is an urgent need to develop potential drugs against coronaviruses. METHODS: High-throughput technology is widely used to explore differences in messenger (m)RNA and micro (mi)RNA expression profiles, especially to investigate protein-protein interactions and search for new therapeutic compounds. We integrated miRNA and mRNA expression profiles in MERS-CoV-infected cells and compared them to mock-infected controls from public databases. RESULTS: Through the bioinformatics analysis, there were 251 upregulated genes and eight highly differentiated miRNAs that overlapped in the two datasets. External validation verified that these genes had high expression in MERS-CoV-infected cells, including RC3H1, NF-kappaB, CD69, TNFAIP3, LEAP-2, DUSP10, CREB5, CXCL2, etc. We revealed that immune, olfactory or sensory system-related, and signal-transduction networks were discovered from upregulated mRNAs in MERS-CoV-infected cells. In total, 115 genes were predicted to be related to miRNAs, with the intersection of upregulated mRNAs and miRNA-targeting prediction genes such as TCF4, NR3C1, and POU2F2. Through the Connectivity Map (CMap) platform, we suggested potential compounds to use against MERS-CoV infection, including diethylcarbamazine, harpagoside, bumetanide, enalapril, and valproic acid. CONCLUSIONS: The present study illustrates the crucial roles of miRNA-mRNA interacting networks in MERS-CoV-infected cells. The genes we identified are potential targets for treating MERS-CoV infection; however, these could possibly be extended to other coronavirus infections. Twit Text FOAVip Gene signatures and potential therapeutic targets of Middle East respiratory syndrome coronavirus (MERS-CoV)-infected human lung adenocarcinoma epithelial cells ????J Microbiol Immunol Infect http://www.kidney.de/pget.php?&tw=1&pmid=34176764 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34176764 #FOAvip English Wikipedia <ref>{{Cite pmid|34176764}}</ref> Gene signatures and potential therapeutic targets of Middle East respiratory syndrome coronavirus (MERS-CoV)-infected human lung adenocarcinoma epithelial cells #MMPMID34176764 Wu YH; Yeh IJ; Phan NN; Yen MC; Hung JH; Chiao CC; Chen CF; Sun Z; Hsu HP; Wang CY; Lai MD J Microbiol Immunol Infect 2021[Oct]; 54 (5): 845-857 PMID34176764show ga BACKGROUND: Pathogenic coronaviruses include Middle East respiratory syndrome coronavirus (MERS-CoV), severe acute respiratory syndrome coronavirus (SARS-CoV), and SARS-CoV-2. These viruses have induced outbreaks worldwide, and there are currently no effective medications against them. Therefore, there is an urgent need to develop potential drugs against coronaviruses. METHODS: High-throughput technology is widely used to explore differences in messenger (m)RNA and micro (mi)RNA expression profiles, especially to investigate protein-protein interactions and search for new therapeutic compounds. We integrated miRNA and mRNA expression profiles in MERS-CoV-infected cells and compared them to mock-infected controls from public databases. RESULTS: Through the bioinformatics analysis, there were 251 upregulated genes and eight highly differentiated miRNAs that overlapped in the two datasets. External validation verified that these genes had high expression in MERS-CoV-infected cells, including RC3H1, NF-kappaB, CD69, TNFAIP3, LEAP-2, DUSP10, CREB5, CXCL2, etc. We revealed that immune, olfactory or sensory system-related, and signal-transduction networks were discovered from upregulated mRNAs in MERS-CoV-infected cells. In total, 115 genes were predicted to be related to miRNAs, with the intersection of upregulated mRNAs and miRNA-targeting prediction genes such as TCF4, NR3C1, and POU2F2. Through the Connectivity Map (CMap) platform, we suggested potential compounds to use against MERS-CoV infection, including diethylcarbamazine, harpagoside, bumetanide, enalapril, and valproic acid. CONCLUSIONS: The present study illustrates the crucial roles of miRNA-mRNA interacting networks in MERS-CoV-infected cells. The genes we identified are potential targets for treating MERS-CoV infection; however, these could possibly be extended to other coronavirus infections. |*Coronavirus Infections[MESH] |Adenocarcinoma of Lung/*virology[MESH] |Antimicrobial Cationic Peptides/genetics/metabolism[MESH] |Blood Proteins/metabolism[MESH] |COVID-19[MESH] |Chemokine CXCL2/genetics/metabolism[MESH] |Cyclic AMP Response Element-Binding Protein A/genetics/metabolism[MESH] |Disease Outbreaks[MESH] |Dual-Specificity Phosphatases/genetics/metabolism[MESH] |Epithelial Cells/*virology[MESH] |Humans[MESH] |Lung Neoplasms/*virology[MESH] |MicroRNAs/genetics/metabolism[MESH] |Middle East Respiratory Syndrome Coronavirus/*genetics/*immunology[MESH] |Mitogen-Activated Protein Kinase Phosphatases/genetics/metabolism[MESH] |Protein Interaction Domains and Motifs[MESH] |SARS-CoV-2[MESH]Gene signatures and potential therapeutic targets of Middle East respi(epmc).pdf DeepDyve Pubget Overpricing