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10.1016/j.jcv.2021.104896 http://scihub22266oqcxt.onion/10.1016/j.jcv.2021.104896 34174710!8196482!34174710     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Virol 2021 ; 141 (ä): 104896 Nephropedia Template TP {{tp|p=34174710|t=2021. One swab, two tests: Validation of dual SARS-CoV-2 testing on the Abbott ID NOW |pdf=|usr=}}{{34174710}} gab.com Text One swab, two tests: Validation of dual SARS-CoV-2 testing on the Abbott ID NOW JO: J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=34174710 #FOAvip BACKGROUND: Point-of-care tests (POCT) are promising tools to detect SARS-CoV-2 in specific settings. Initial reports suggest the ID NOW COVID-19 assay (Abbott Diagnostics Inc, USA) is less sensitive than standard real-time reverse transcription polymerase chain reaction (rRT-PCR) assays. This has raised concern over false negatives in SARS-CoV-2 POCT. OBJECTIVES: We compared the performance of the ID NOW COVID-19 assay to our in-house rRT-PCR assay to assess whether dry swabs used in ID NOW testing could be stored in transport media and be re-tested by rRT-PCR for redundancy and to provide material for further investigation. METHODS: Paired respiratory swabs collected from patients at three acute care hospitals were used. One swab in transport media (McMaster Molecular Media (MMM)) was tested for SARS-CoV-2 by a laboratory-developed two-target rRT-PCR assay. The second was stored dry in a sterile container and tested by the ID NOW COVID-19 assay. Following ID NOW testing, dry swabs were stored in MMM for up to 48 h and re-tested by rRT-PCR. Serially diluted SARS-CoV-2 particles were used to assess the impact of heat inactivation and storage time. RESULTS: Respiratory swabs (n = 343) from 179 individuals were included. Using rRT-PCR results as the comparator, the ID NOW COVID-19 assay had positive (PPA) and negative (NPA) percent agreements of 87.0% (95% CI:0.74-0.94) and 99.7% (95% CI:0.98-0.99). Re-tested swabs placed in MMM following ID NOW testing had PPA and NPA of 88.8% (95% CI:0.76-0.95) and 99.7% (95% CI:0.98-0.99), respectively. CONCLUSIONS: Storing spent dry swabs in transport media for redundancy rRT-PCR testing is a potential approach to address possible false negatives with the ID NOW COVID-19 assay. Twit Text FOAVip One swab, two tests: Validation of dual SARS-CoV-2 testing on the Abbott ID NOW ????J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=34174710 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34174710 #FOAvip English Wikipedia <ref>{{Cite pmid|34174710}}</ref> One swab, two tests: Validation of dual SARS-CoV-2 testing on the Abbott ID NOW #MMPMID34174710 Burnes LE; Clark ST; Sheldrake E; Faheem A; Poon BP; Christie-Holmes N; Finlay L; Kandel C; Phan M; Frankland C; Lau T; Gubbay JB; Corbeil A; Katz K; Kozak RA J Clin Virol 2021[Aug]; 141 (ä): 104896 PMID34174710show ga BACKGROUND: Point-of-care tests (POCT) are promising tools to detect SARS-CoV-2 in specific settings. Initial reports suggest the ID NOW COVID-19 assay (Abbott Diagnostics Inc, USA) is less sensitive than standard real-time reverse transcription polymerase chain reaction (rRT-PCR) assays. This has raised concern over false negatives in SARS-CoV-2 POCT. OBJECTIVES: We compared the performance of the ID NOW COVID-19 assay to our in-house rRT-PCR assay to assess whether dry swabs used in ID NOW testing could be stored in transport media and be re-tested by rRT-PCR for redundancy and to provide material for further investigation. METHODS: Paired respiratory swabs collected from patients at three acute care hospitals were used. One swab in transport media (McMaster Molecular Media (MMM)) was tested for SARS-CoV-2 by a laboratory-developed two-target rRT-PCR assay. The second was stored dry in a sterile container and tested by the ID NOW COVID-19 assay. Following ID NOW testing, dry swabs were stored in MMM for up to 48 h and re-tested by rRT-PCR. Serially diluted SARS-CoV-2 particles were used to assess the impact of heat inactivation and storage time. RESULTS: Respiratory swabs (n = 343) from 179 individuals were included. Using rRT-PCR results as the comparator, the ID NOW COVID-19 assay had positive (PPA) and negative (NPA) percent agreements of 87.0% (95% CI:0.74-0.94) and 99.7% (95% CI:0.98-0.99). Re-tested swabs placed in MMM following ID NOW testing had PPA and NPA of 88.8% (95% CI:0.76-0.95) and 99.7% (95% CI:0.98-0.99), respectively. CONCLUSIONS: Storing spent dry swabs in transport media for redundancy rRT-PCR testing is a potential approach to address possible false negatives with the ID NOW COVID-19 assay. |*COVID-19[MESH] |*SARS-CoV-2[MESH] |COVID-19 Testing[MESH] |Humans[MESH] |Point-of-Care Testing[MESH] |Sensitivity and Specificity[MESH]One swab, two tests: Validation of dual SARS-CoV-2 testing on the Abbo(epmc).pdf DeepDyve Pubget Overpricing