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10.1016/j.cell.2021.05.046 http://scihub22266oqcxt.onion/10.1016/j.cell.2021.05.046 34171305!8173604!34171305     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell 2021 ; 184 (15): 3962-3980.e17 Nephropedia Template TP {{tp|p=34171305|t=2021. Profiling SARS-CoV-2 HLA-I peptidome reveals T cell epitopes from out-of-frame ORFs |pdf=|usr=}}{{34171305}} gab.com Text Profiling SARS-CoV-2 HLA-I peptidome reveals T cell epitopes from out-of-frame ORFs JO: Cell http://www.kidney.de/pget.php?&tw=1&pmid=34171305 #FOAvip T cell-mediated immunity plays an important role in controlling SARS-CoV-2 infection, but the repertoire of naturally processed and presented viral epitopes on class I human leukocyte antigen (HLA-I) remains uncharacterized. Here, we report the first HLA-I immunopeptidome of SARS-CoV-2 in two cell lines at different times post infection using mass spectrometry. We found HLA-I peptides derived not only from canonical open reading frames (ORFs) but also from internal out-of-frame ORFs in spike and nucleocapsid not captured by current vaccines. Some peptides from out-of-frame ORFs elicited T cell responses in a humanized mouse model and individuals with COVID-19 that exceeded responses to canonical peptides, including some of the strongest epitopes reported to date. Whole-proteome analysis of infected cells revealed that early expressed viral proteins contribute more to HLA-I presentation and immunogenicity. These biological insights, as well as the discovery of out-of-frame ORF epitopes, will facilitate selection of peptides for immune monitoring and vaccine development. Twit Text FOAVip Profiling SARS-CoV-2 HLA-I peptidome reveals T cell epitopes from out-of-frame ORFs ????Cell http://www.kidney.de/pget.php?&tw=1&pmid=34171305 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34171305 #FOAvip English Wikipedia <ref>{{Cite pmid|34171305}}</ref> Profiling SARS-CoV-2 HLA-I peptidome reveals T cell epitopes from out-of-frame ORFs #MMPMID34171305 Weingarten-Gabbay S; Klaeger S; Sarkizova S; Pearlman LR; Chen DY; Gallagher KME; Bauer MR; Taylor HB; Dunn WA; Tarr C; Sidney J; Rachimi S; Conway HL; Katsis K; Wang Y; Leistritz-Edwards D; Durkin MR; Tomkins-Tinch CH; Finkel Y; Nachshon A; Gentili M; Rivera KD; Carulli IP; Chea VA; Chandrashekar A; Bozkus CC; Carrington M; Bhardwaj N; Barouch DH; Sette A; Maus MV; Rice CM; Clauser KR; Keskin DB; Pregibon DC; Hacohen N; Carr SA; Abelin JG; Saeed M; Sabeti PC Cell 2021[Jul]; 184 (15): 3962-3980.e17 PMID34171305show ga T cell-mediated immunity plays an important role in controlling SARS-CoV-2 infection, but the repertoire of naturally processed and presented viral epitopes on class I human leukocyte antigen (HLA-I) remains uncharacterized. Here, we report the first HLA-I immunopeptidome of SARS-CoV-2 in two cell lines at different times post infection using mass spectrometry. We found HLA-I peptides derived not only from canonical open reading frames (ORFs) but also from internal out-of-frame ORFs in spike and nucleocapsid not captured by current vaccines. Some peptides from out-of-frame ORFs elicited T cell responses in a humanized mouse model and individuals with COVID-19 that exceeded responses to canonical peptides, including some of the strongest epitopes reported to date. Whole-proteome analysis of infected cells revealed that early expressed viral proteins contribute more to HLA-I presentation and immunogenicity. These biological insights, as well as the discovery of out-of-frame ORF epitopes, will facilitate selection of peptides for immune monitoring and vaccine development. |A549 Cells[MESH] |Alleles[MESH] |Amino Acid Sequence[MESH] |Animals[MESH] |Antigen Presentation/immunology[MESH] |COVID-19/immunology/virology[MESH] |Epitopes, T-Lymphocyte/*immunology[MESH] |Female[MESH] |HEK293 Cells[MESH] |Histocompatibility Antigens Class I/*immunology[MESH] |Humans[MESH] |Kinetics[MESH] |Male[MESH] |Mice[MESH] |Open Reading Frames/*genetics[MESH] |Peptides/chemistry/*immunology[MESH] |Proteome/*immunology[MESH] |SARS-CoV-2/*immunology[MESH]Profiling SARS-CoV-2 HLA-I peptidome reveals T cell epitopes from out-(epmc).pdf DeepDyve Pubget Overpricing