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10.1038/s41589-021-00832-4 http://scihub22266oqcxt.onion/10.1038/s41589-021-00832-4 34168368!8463502!34168368     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Chem+Biol 2021 ; 17 (10): 1057-1064 Nephropedia Template TP {{tp|p=34168368|t=2021. Rapid generation of potent antibodies by autonomous hypermutation in yeast |pdf=|usr=}}{{34168368}} gab.com Text Rapid generation of potent antibodies by autonomous hypermutation in yeast JO: Nat Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=34168368 #FOAvip The predominant approach for antibody generation remains animal immunization, which can yield exceptionally selective and potent antibody clones owing to the powerful evolutionary process of somatic hypermutation. However, animal immunization is inherently slow, not always accessible and poorly compatible with many antigens. Here, we describe 'autonomous hypermutation yeast surface display' (AHEAD), a synthetic recombinant antibody generation technology that imitates somatic hypermutation inside engineered yeast. By encoding antibody fragments on an error-prone orthogonal DNA replication system, surface-displayed antibody repertoires continuously mutate through simple cycles of yeast culturing and enrichment for antigen binding to produce high-affinity clones in as little as two weeks. We applied AHEAD to generate potent nanobodies against the SARS-CoV-2 S glycoprotein, a G-protein-coupled receptor and other targets, offering a template for streamlined antibody generation at large. Twit Text FOAVip Rapid generation of potent antibodies by autonomous hypermutation in yeast ????Nat Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=34168368 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34168368 #FOAvip English Wikipedia <ref>{{Cite pmid|34168368}}</ref> Rapid generation of potent antibodies by autonomous hypermutation in yeast #MMPMID34168368 Wellner A; McMahon C; Gilman MSA; Clements JR; Clark S; Nguyen KM; Ho MH; Hu VJ; Shin JE; Feldman J; Hauser BM; Caradonna TM; Wingler LM; Schmidt AG; Marks DS; Abraham J; Kruse AC; Liu CC Nat Chem Biol 2021[Oct]; 17 (10): 1057-1064 PMID34168368show ga The predominant approach for antibody generation remains animal immunization, which can yield exceptionally selective and potent antibody clones owing to the powerful evolutionary process of somatic hypermutation. However, animal immunization is inherently slow, not always accessible and poorly compatible with many antigens. Here, we describe 'autonomous hypermutation yeast surface display' (AHEAD), a synthetic recombinant antibody generation technology that imitates somatic hypermutation inside engineered yeast. By encoding antibody fragments on an error-prone orthogonal DNA replication system, surface-displayed antibody repertoires continuously mutate through simple cycles of yeast culturing and enrichment for antigen binding to produce high-affinity clones in as little as two weeks. We applied AHEAD to generate potent nanobodies against the SARS-CoV-2 S glycoprotein, a G-protein-coupled receptor and other targets, offering a template for streamlined antibody generation at large. |Antibodies/immunology[MESH] |Antibody Formation/*immunology[MESH] |Antigens[MESH] |COVID-19/immunology[MESH] |Humans[MESH] |Peptide Library[MESH] |Protein Engineering/*methods[MESH] |Recombinant Proteins/*biosynthesis/metabolism[MESH] |SARS-CoV-2/immunology/pathogenicity[MESH] |Saccharomyces cerevisiae/metabolism[MESH] |Single-Domain Antibodies/genetics/metabolism[MESH]Rapid generation of potent antibodies by autonomous hypermutation in y(epmc).pdf DeepDyve Pubget Overpricing