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10.1016/j.bios.2021.113406

http://scihub22266oqcxt.onion/10.1016/j.bios.2021.113406
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34167074!8178056!34167074
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suck abstract from ncbi


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pmid34167074      Biosens+Bioelectron 2021 ; 191 (ä): 113406
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  • A rapid quantitative on-site coronavirus disease 19 serological test #MMPMID34167074
  • Lee JH; Bae PK; Kim H; Song YJ; Yi SY; Kwon J; Seo JS; Lee JM; Jo HS; Park SM; Park HS; Shin KS; Chung S; Shin YB
  • Biosens Bioelectron 2021[Nov]; 191 (ä): 113406 PMID34167074show ga
  • On-site severe acute respiratory syndrome coronavirus 2 (SARS CoV-2) serological assays allow for timely in-field decisions to be made regarding patient status, also enabling population-wide screening to assist in controlling the coronavirus disease 2019 (COVID-19) pandemic. Here we propose a rapid microfluidic serological assay with two unique functions of nanointerstice filling and digitized flow control, which enable the fast/robust filling of the sample fluid as well as precise regulation of duration and volume of immune reaction. Developed microfluidic assay showed enhanced limit of detection, and 91.67% sensitivity and 100% specificity (n = 152) for clinical samples of SARS CoV-2 patients. The assay enables daily monitoring of IgM/IgG titers and patterns, which could be crucial parameters for convalescence from COVID-19 and provide important insight into how the immune system responds to SARS CoV-2. The developed on-site microfluidic assay presented the mean time for IgM and IgG seroconversions, indicating that these titers plateaued days after seroconversion. The mean duration from day 0 to PCR negativity was 19.4 days (median 20 d, IQR 16-21 d), with higher IgM/IgG titres being observed when PCR positive turns into negative. Simple monitoring of these titres promotes rapid on-site detection and comprehensive understanding of the immune response of COVID-19 patients.
  • |*Biosensing Techniques[MESH]
  • |*COVID-19[MESH]
  • |Antibodies, Viral[MESH]
  • |Humans[MESH]
  • |Immunoassay[MESH]
  • |Immunoglobulin G[MESH]
  • |Immunoglobulin M[MESH]
  • |SARS-CoV-2[MESH]
  • |Sensitivity and Specificity[MESH]


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