Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Cell+Rep 2021 ; 35 (13): 109305 Nephropedia Template TP
gab.com Text
Twit Text FOAVip
Twit Text #
English Wikipedia
Identification of presented SARS-CoV-2 HLA class I and HLA class II peptides using HLA peptidomics #MMPMID34166618
Nagler A; Kalaora S; Barbolin C; Gangaev A; Ketelaars SLC; Alon M; Pai J; Benedek G; Yahalom-Ronen Y; Erez N; Greenberg P; Yagel G; Peri A; Levin Y; Satpathy AT; Bar-Haim E; Paran N; Kvistborg P; Samuels Y
Cell Rep 2021[Jun]; 35 (13): 109305 PMID34166618show ga
The human leukocyte antigen (HLA)-bound viral antigens serve as an immunological signature that can be selectively recognized by T cells. As viruses evolve by acquiring mutations, it is essential to identify a range of presented viral antigens. Using HLA peptidomics, we are able to identify severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-derived peptides presented by highly prevalent HLA class I (HLA-I) molecules by using infected cells as well as overexpression of SARS-CoV-2 genes. We find 26 HLA-I peptides and 36 HLA class II (HLA-II) peptides. Among the identified peptides, some are shared between different cells and some are derived from out-of-frame open reading frames (ORFs). Seven of these peptides were previously shown to be immunogenic, and we identify two additional immunoreactive peptides by using HLA multimer staining. These results may aid the development of the next generation of SARS-CoV-2 vaccines based on presented viral-specific antigens that span several of the viral genes.
|Antigen Presentation[MESH]
|Antigens, Viral/*immunology/metabolism[MESH]
|COVID-19 Vaccines[MESH]
|COVID-19/*immunology/*virology[MESH]
|Cell Line[MESH]
|Epitopes, T-Lymphocyte/immunology[MESH]
|HEK293 Cells[MESH]
|Histocompatibility Antigens Class I/immunology[MESH]
|Histocompatibility Antigens Class II/immunology[MESH]