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10.1371/journal.ppat.1009683 http://scihub22266oqcxt.onion/10.1371/journal.ppat.1009683 34166473!8263067!34166473     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+Pathog 2021 ; 17 (6): e1009683 Nephropedia Template TP {{tp|p=34166473|t=2021. Rapid, reliable, and reproducible cell fusion assay to quantify SARS-Cov-2 spike interaction with hACE2 |pdf=|usr=}}{{34166473}} gab.com Text Rapid, reliable, and reproducible cell fusion assay to quantify SARS-Cov-2 spike interaction with hACE2 JO: PLoS Pathog http://www.kidney.de/pget.php?&tw=1&pmid=34166473 #FOAvip COVID-19 is a global crisis of unimagined dimensions. Currently, Remedesivir is only fully licensed FDA therapeutic. A major target of the vaccine effort is the SARS-CoV-2 spike-hACE2 interaction, and assessment of efficacy relies on time consuming neutralization assay. Here, we developed a cell fusion assay based upon spike-hACE2 interaction. The system was tested by transient co-transfection of 293T cells, which demonstrated good correlation with standard spike pseudotyping for inhibition by sera and biologics. Then established stable cell lines were very well behaved and gave even better correlation with pseudotyping results, after a short, overnight co-incubation. Results with the stable cell fusion assay also correlated well with those of a live virus assay. In summary we have established a rapid, reliable, and reproducible cell fusion assay that will serve to complement the other neutralization assays currently in use, is easy to implement in most laboratories, and may serve as the basis for high throughput screens to identify inhibitors of SARS-CoV-2 virus-cell binding and entry. Twit Text FOAVip Rapid, reliable, and reproducible cell fusion assay to quantify SARS-Cov-2 spike interaction with hACE2 ????PLoS Pathog http://www.kidney.de/pget.php?&tw=1&pmid=34166473 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34166473 #FOAvip English Wikipedia <ref>{{Cite pmid|34166473}}</ref> Rapid, reliable, and reproducible cell fusion assay to quantify SARS-Cov-2 spike interaction with hACE2 #MMPMID34166473 Zhao M; Su PY; Castro DA; Tripler TN; Hu Y; Cook M; Ko AI; Farhadian SF; Israelow B; Dela Cruz CS; Xiong Y; Sutton RE PLoS Pathog 2021[Jun]; 17 (6): e1009683 PMID34166473show ga COVID-19 is a global crisis of unimagined dimensions. Currently, Remedesivir is only fully licensed FDA therapeutic. A major target of the vaccine effort is the SARS-CoV-2 spike-hACE2 interaction, and assessment of efficacy relies on time consuming neutralization assay. Here, we developed a cell fusion assay based upon spike-hACE2 interaction. The system was tested by transient co-transfection of 293T cells, which demonstrated good correlation with standard spike pseudotyping for inhibition by sera and biologics. Then established stable cell lines were very well behaved and gave even better correlation with pseudotyping results, after a short, overnight co-incubation. Results with the stable cell fusion assay also correlated well with those of a live virus assay. In summary we have established a rapid, reliable, and reproducible cell fusion assay that will serve to complement the other neutralization assays currently in use, is easy to implement in most laboratories, and may serve as the basis for high throughput screens to identify inhibitors of SARS-CoV-2 virus-cell binding and entry. |Angiotensin-Converting Enzyme 2/genetics/*metabolism[MESH] |Biological Assay/*methods[MESH] |COVID-19/blood/*virology[MESH] |Cell Fusion[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Receptors, Coronavirus/genetics/*metabolism[MESH] |SARS-CoV-2/*physiology[MESH] |Spike Glycoprotein, Coronavirus/genetics/*metabolism[MESH] |Transfection[MESH]Rapid, reliable, and reproducible cell fusion assay to quantify SARS-C(epmc).pdf DeepDyve Pubget Overpricing