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10.3389/fcimb.2021.685640 http://scihub22266oqcxt.onion/10.3389/fcimb.2021.685640 34164346!8216554!34164346     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34164346&cmd=llinks): Failed to open stream: HTTP request failed! 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Assessment of the Diagnostic Ability of Four Detection Methods Using Three Sample Types of COVID-19 Patients |pdf=|usr=}}{{34164346}} gab.com Text Assessment of the Diagnostic Ability of Four Detection Methods Using Three Sample Types of COVID-19 Patients JO: Front Cell Infect Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=34164346 #FOAvip BACKGROUND: Viral nucleic acid detection is considered the gold standard for the diagnosis of coronavirus disease 2019 (COVID-19), which is caused by SARS-CoV-2 infection. However, unsuitable sample types and laboratory detection kits/methods lead to misdiagnosis, which delays the prevention and control of the pandemic. METHODS: We compared four nucleic acid detection methods [two kinds of reverse transcription polymerase chain reactions (RT-PCR A: ORF1ab and N testing; RT-PCRB: only ORF1ab testing), reverse transcription recombinase aided amplification (RT-RAA) and droplet digital RT-PCR (dd-RT-PCR)] using 404 samples of 72 hospitalized COVID-19 patients, including oropharyngeal swab (OPS), nasopharyngeal swabs (NPS) and saliva after deep cough, to evaluate the best sample type and method for SARS-CoV-2 detection. RESULTS: Among the four methods, dd-RT-PCR exhibited the highest positivity rate (93.0%), followed by RT-PCR B (91.2%) and RT-RAA (91.2%), while the positivity rate of RT-PCR A was only 71.9%. The viral load in OPS [24.90 copies/test (IQR 15.58-129.85)] was significantly lower than that in saliva [292.30 copies/test (IQR 20.20-8628.55)] and NPS [274.40 copies/test (IQR 33.10-2836.45)]. In addition, if OPS samples were tested alone by RT-PCR A, only 21.4% of the COVID-19 patients would be considered positive. The accuracy of all methods reached nearly 100% when saliva and NPS samples from the same patient were tested simultaneously. CONCLUSIONS: SARS-CoV-2 nucleic acid detection methods should be fully evaluated before use. High-positivity rate methods such as RT-RAA and dd-RT-PCR should be considered when possible. Furthermore, saliva after deep cough and NPS can greatly improve the accuracy of the diagnosis, and testing OPS alone is not recommended. Twit Text FOAVip Assessment of the Diagnostic Ability of Four Detection Methods Using Three Sample Types of COVID-19 Patients ????Front Cell Infect Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=34164346 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34164346 #FOAvip English Wikipedia <ref>{{Cite pmid|34164346}}</ref> Assessment of the Diagnostic Ability of Four Detection Methods Using Three Sample Types of COVID-19 Patients #MMPMID34164346 Yu F; Xie G; Zheng S; Han D; Bao J; Zhang D; Feng B; Wang Q; Zou Q; Wang R; Yang X; Chen W; Lou B; Chen Y Front Cell Infect Microbiol 2021[]; 11 (ä): 685640 PMID34164346show ga BACKGROUND: Viral nucleic acid detection is considered the gold standard for the diagnosis of coronavirus disease 2019 (COVID-19), which is caused by SARS-CoV-2 infection. However, unsuitable sample types and laboratory detection kits/methods lead to misdiagnosis, which delays the prevention and control of the pandemic. METHODS: We compared four nucleic acid detection methods [two kinds of reverse transcription polymerase chain reactions (RT-PCR A: ORF1ab and N testing; RT-PCRB: only ORF1ab testing), reverse transcription recombinase aided amplification (RT-RAA) and droplet digital RT-PCR (dd-RT-PCR)] using 404 samples of 72 hospitalized COVID-19 patients, including oropharyngeal swab (OPS), nasopharyngeal swabs (NPS) and saliva after deep cough, to evaluate the best sample type and method for SARS-CoV-2 detection. RESULTS: Among the four methods, dd-RT-PCR exhibited the highest positivity rate (93.0%), followed by RT-PCR B (91.2%) and RT-RAA (91.2%), while the positivity rate of RT-PCR A was only 71.9%. The viral load in OPS [24.90 copies/test (IQR 15.58-129.85)] was significantly lower than that in saliva [292.30 copies/test (IQR 20.20-8628.55)] and NPS [274.40 copies/test (IQR 33.10-2836.45)]. In addition, if OPS samples were tested alone by RT-PCR A, only 21.4% of the COVID-19 patients would be considered positive. The accuracy of all methods reached nearly 100% when saliva and NPS samples from the same patient were tested simultaneously. CONCLUSIONS: SARS-CoV-2 nucleic acid detection methods should be fully evaluated before use. High-positivity rate methods such as RT-RAA and dd-RT-PCR should be considered when possible. Furthermore, saliva after deep cough and NPS can greatly improve the accuracy of the diagnosis, and testing OPS alone is not recommended. |*COVID-19/diagnosis[MESH] |COVID-19 Nucleic Acid Testing[MESH] |COVID-19 Testing/*methods[MESH] |Humans[MESH] |Nasopharynx[MESH] |Pandemics[MESH] |RNA, Viral/genetics[MESH] |SARS-CoV-2[MESH] |Saliva[MESH]Assessment of the Diagnostic Ability of Four Detection Methods Using T(epmc).pdf DeepDyve Pubget Overpricing