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10.1039/d0sc04978f http://scihub22266oqcxt.onion/10.1039/d0sc04978f 34163622!8179461!34163622     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34163622&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Chem+Sci 2021 ; 12 (10): 3489-3496 Nephropedia Template TP {{tp|p=34163622|t=2021. A microscopic description of SARS-CoV-2 main protease inhibition with Michael acceptors Strategies for improving inhibitor design |pdf=|usr=}}{{34163622}} gab.com Text A microscopic description of SARS-CoV-2 main protease inhibition with Michael acceptors Strategies for improving inhibitor design JO: Chem Sci http://www.kidney.de/pget.php?&tw=1&pmid=34163622 #FOAvip The irreversible inhibition of the main protease of SARS-CoV-2 by a Michael acceptor known as N3 has been investigated using multiscale methods. The noncovalent enzyme-inhibitor complex was simulated using classical molecular dynamics techniques and the pose of the inhibitor in the active site was compared to that of the natural substrate, a peptide containing the Gln-Ser scissile bond. The formation of the covalent enzyme-inhibitor complex was then simulated using hybrid QM/MM free energy methods. After binding, the reaction mechanism was found to be composed of two steps: (i) the activation of the catalytic dyad (Cys145 and His41) to form an ion pair and (ii) a Michael addition where the attack of the Sgamma atom of Cys145 to the Cbeta atom of the inhibitor precedes the water-mediated proton transfer from His41 to the Calpha atom. The microscopic description of protease inhibition by N3 obtained from our simulations is strongly supported by the excellent agreement between the estimated activation free energy and the value derived from kinetic experiments. Comparison with the acylation reaction of a peptide substrate suggests that N3-based inhibitors could be improved by adding chemical modifications that could facilitate the formation of the catalytic dyad ion pair. Twit Text FOAVip A microscopic description of SARS-CoV-2 main protease inhibition with Michael acceptors Strategies for improving inhibitor design ????Chem Sci http://www.kidney.de/pget.php?&tw=1&pmid=34163622 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34163622 #FOAvip English Wikipedia <ref>{{Cite pmid|34163622}}</ref> A microscopic description of SARS-CoV-2 main protease inhibition with Michael acceptors Strategies for improving inhibitor design #MMPMID34163622 Ramos-Guzman CA; Ruiz-Pernia JJ; Tunon I Chem Sci 2021[Jan]; 12 (10): 3489-3496 PMID34163622show ga The irreversible inhibition of the main protease of SARS-CoV-2 by a Michael acceptor known as N3 has been investigated using multiscale methods. The noncovalent enzyme-inhibitor complex was simulated using classical molecular dynamics techniques and the pose of the inhibitor in the active site was compared to that of the natural substrate, a peptide containing the Gln-Ser scissile bond. The formation of the covalent enzyme-inhibitor complex was then simulated using hybrid QM/MM free energy methods. After binding, the reaction mechanism was found to be composed of two steps: (i) the activation of the catalytic dyad (Cys145 and His41) to form an ion pair and (ii) a Michael addition where the attack of the Sgamma atom of Cys145 to the Cbeta atom of the inhibitor precedes the water-mediated proton transfer from His41 to the Calpha atom. The microscopic description of protease inhibition by N3 obtained from our simulations is strongly supported by the excellent agreement between the estimated activation free energy and the value derived from kinetic experiments. Comparison with the acylation reaction of a peptide substrate suggests that N3-based inhibitors could be improved by adding chemical modifications that could facilitate the formation of the catalytic dyad ion pair. ?A microscopic description of SARS-CoV-2 main protease inhibition with (epmc).pdf DeepDyve Pubget Overpricing