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10.1038/s41467-021-24078-9 http://scihub22266oqcxt.onion/10.1038/s41467-021-24078-9 34162869!8222401!34162869     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2021 ; 12 (1): 3903 Nephropedia Template TP {{tp|p=34162869|t=2021. COVseq is a cost-effective workflow for mass-scale SARS-CoV-2 genomic surveillance |pdf=|usr=}}{{34162869}} gab.com Text COVseq is a cost-effective workflow for mass-scale SARS-CoV-2 genomic surveillance JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=34162869 #FOAvip While mass-scale vaccination campaigns are ongoing worldwide, genomic surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical to monitor the emergence and global spread of viral variants of concern (VOC). Here, we present a streamlined workflow-COVseq-which can be used to generate highly multiplexed sequencing libraries compatible with Illumina platforms from hundreds of SARS-CoV-2 samples in parallel, in a rapid and cost-effective manner. We benchmark COVseq against a standard library preparation method (NEBNext) on 29 SARS-CoV-2 positive samples, reaching 95.4% of concordance between single-nucleotide variants detected by both methods. Application of COVseq to 245 additional SARS-CoV-2 positive samples demonstrates the ability of the method to reliably detect emergent VOC as well as its compatibility with downstream phylogenetic analyses. A cost analysis shows that COVseq could be used to sequence thousands of samples at less than 15 USD per sample, including library preparation and sequencing costs. We conclude that COVseq is a versatile and scalable method that is immediately applicable for SARS-CoV-2 genomic surveillance and easily adaptable to other pathogens such as influenza viruses. Twit Text FOAVip COVseq is a cost-effective workflow for mass-scale SARS-CoV-2 genomic surveillance ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=34162869 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34162869 #FOAvip English Wikipedia <ref>{{Cite pmid|34162869}}</ref> COVseq is a cost-effective workflow for mass-scale SARS-CoV-2 genomic surveillance #MMPMID34162869 Simonetti M; Zhang N; Harbers L; Milia MG; Brossa S; Huong Nguyen TT; Cerutti F; Berrino E; Sapino A; Bienko M; Sottile A; Ghisetti V; Crosetto N Nat Commun 2021[Jun]; 12 (1): 3903 PMID34162869show ga While mass-scale vaccination campaigns are ongoing worldwide, genomic surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical to monitor the emergence and global spread of viral variants of concern (VOC). Here, we present a streamlined workflow-COVseq-which can be used to generate highly multiplexed sequencing libraries compatible with Illumina platforms from hundreds of SARS-CoV-2 samples in parallel, in a rapid and cost-effective manner. We benchmark COVseq against a standard library preparation method (NEBNext) on 29 SARS-CoV-2 positive samples, reaching 95.4% of concordance between single-nucleotide variants detected by both methods. Application of COVseq to 245 additional SARS-CoV-2 positive samples demonstrates the ability of the method to reliably detect emergent VOC as well as its compatibility with downstream phylogenetic analyses. A cost analysis shows that COVseq could be used to sequence thousands of samples at less than 15 USD per sample, including library preparation and sequencing costs. We conclude that COVseq is a versatile and scalable method that is immediately applicable for SARS-CoV-2 genomic surveillance and easily adaptable to other pathogens such as influenza viruses. |Animals[MESH] |COVID-19/blood/economics/*genetics/virology[MESH] |Cells, Cultured[MESH] |Chlorocebus aethiops[MESH] |Cost-Benefit Analysis[MESH] |Epidemiological Monitoring[MESH] |Genome, Viral[MESH] |High-Throughput Nucleotide Sequencing/economics/methods[MESH] |Humans[MESH] |Phylogeny[MESH] |SARS-CoV-2/*genetics/isolation & purification/pathogenicity[MESH]COVseq is a cost-effective workflow for mass-scale SARS-CoV-2 genomic (epmc).pdf DeepDyve Pubget Overpricing