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10.1016/j.ijid.2021.06.031

http://scihub22266oqcxt.onion/10.1016/j.ijid.2021.06.031
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suck abstract from ncbi


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pmid34161801      Int+J+Infect+Dis 2021 ; 109 (ä): 85-89
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  • Comparison of two assays to detect IgG antibodies to the receptor binding domain of SARS?CoV?2 as a surrogate marker for assessing neutralizing antibodies in COVID-19 patients #MMPMID34161801
  • Kamaladasa A; Gunasekara B; Jeewandara C; Jayathilaka D; Wijewickrama A; Guruge D; Wijayamuni R; Tan TK; Ogg GS; Townsend A; Malavige GN
  • Int J Infect Dis 2021[Aug]; 109 (ä): 85-89 PMID34161801show ga
  • BACKGROUND: Neutralizing antibodies (NAbs) are important for protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reinfection. In this study, two assays that are correlated with NAbs were compared: the haemagglutination test (HAT) and the surrogate virus neutralization test (sVNT). METHODS: The specificity of the HAT was compared with the sVNT, and the sensitivity and persistence of antibodies in patients with varying severity of illness was assessed in a cohort of 71 patients at 4-6 weeks and 13-16 weeks. The kinetics were assessed in the first, second, and third weeks in patients with varying severity of acute illness. RESULTS: The specificity of the HAT was >99%, and sensitivity was similar to the sVNT. The levels of HAT were significantly and positively correlated with those of the sVNT (Spearman's r = 0.78, P < 0.0001). Patients with moderate and severe illness had higher HAT titres when compared to those with mild illness. Six of seven patients with severe illness had a titre of >1:640 during the second week of illness, whereas only five of 31 patients with a mild illness had a titre of >1:160 in the second week of illness. CONCLUSIONS: Since the HAT is a simple and very cheap assay to perform, it would be ideal to use as an indicator of NAbs in resource-poor settings.
  • |*COVID-19[MESH]
  • |*SARS-CoV-2[MESH]
  • |Antibodies, Neutralizing[MESH]
  • |Antibodies, Viral[MESH]
  • |Biomarkers[MESH]
  • |Humans[MESH]
  • |Immunoglobulin G[MESH]


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