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10.1167/iovs.62.7.25 http://scihub22266oqcxt.onion/10.1167/iovs.62.7.25 34160563!8237080!34160563     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Invest+Ophthalmol+Vis+Sci 2021 ; 62 (7): 25 Nephropedia Template TP {{tp|p=34160563|t=2021. ACE2 Expression Is Upregulated in Inflammatory Corneal Epithelial Cells and Attenuated by Resveratrol |pdf=|usr=}}{{34160563}} gab.com Text ACE2 Expression Is Upregulated in Inflammatory Corneal Epithelial Cells and Attenuated by Resveratrol JO: Invest Ophthalmol Vis Sci http://www.kidney.de/pget.php?&tw=1&pmid=34160563 #FOAvip PURPOSE: The ocular surface is considered an important route for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission. The expression level of the SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) is vital for viral infection. However, the regulation of ACE2 expression on the ocular surface is still unknown. We aimed to determine the change in ACE2 expression in inflamed corneal epithelium and explore potential drugs to reduce the expression of ACE2 on the ocular surface. METHODS: The expression of the SARS-CoV-2 receptors ACE2 and TMPRSS2 in human corneal epithelial cells (HCECs) was examined by qPCR and Western blotting. The altered expression of ACE2 in inflammatory corneal epithelium was evaluated in TNFalpha- and IL-1beta-stimulated HCECs and inflamed mouse corneal epithelium, and the effect of resveratrol on ACE2 expression in HCECs was detected by immunofluorescence and Western blot analysis. RESULTS: ACE2 and TMPRSS2 are expressed on the human corneal epithelial cells. ACE2 expression is upregulated in HCECs by stimulation with TNFalpha and IL-1beta and inflamed mouse corneas, including dry eye and alkali-burned corneas. In addition, resveratrol attenuates the increased expression of ACE2 induced by TNFalpha in HCECs. CONCLUSIONS: This study demonstrates that ACE2 is highly expressed in HCECs and can be upregulated by stimulation with inflammatory cytokines and inflamed mouse corneal epithelium. Resveratrol may be able to reduce the increased expression of ACE2 on the inflammatory ocular surface. Our work suggests that patients with an inflammatory ocular surface may display higher ACE2 expression, which increases the risk of SARS-CoV-2 infection. Twit Text FOAVip ACE2 Expression Is Upregulated in Inflammatory Corneal Epithelial Cells and Attenuated by Resveratrol ????Invest Ophthalmol Vis Sci http://www.kidney.de/pget.php?&tw=1&pmid=34160563 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34160563 #FOAvip English Wikipedia <ref>{{Cite pmid|34160563}}</ref> ACE2 Expression Is Upregulated in Inflammatory Corneal Epithelial Cells and Attenuated by Resveratrol #MMPMID34160563 Jiang Z; Zhang H; Gao J; Yu H; Han R; Zhu L; Chen X; Fan Q; Hao P; Wang L; Li X Invest Ophthalmol Vis Sci 2021[Jun]; 62 (7): 25 PMID34160563show ga PURPOSE: The ocular surface is considered an important route for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission. The expression level of the SARS-CoV-2 receptor angiotensin-converting enzyme 2 (ACE2) is vital for viral infection. However, the regulation of ACE2 expression on the ocular surface is still unknown. We aimed to determine the change in ACE2 expression in inflamed corneal epithelium and explore potential drugs to reduce the expression of ACE2 on the ocular surface. METHODS: The expression of the SARS-CoV-2 receptors ACE2 and TMPRSS2 in human corneal epithelial cells (HCECs) was examined by qPCR and Western blotting. The altered expression of ACE2 in inflammatory corneal epithelium was evaluated in TNFalpha- and IL-1beta-stimulated HCECs and inflamed mouse corneal epithelium, and the effect of resveratrol on ACE2 expression in HCECs was detected by immunofluorescence and Western blot analysis. RESULTS: ACE2 and TMPRSS2 are expressed on the human corneal epithelial cells. ACE2 expression is upregulated in HCECs by stimulation with TNFalpha and IL-1beta and inflamed mouse corneas, including dry eye and alkali-burned corneas. In addition, resveratrol attenuates the increased expression of ACE2 induced by TNFalpha in HCECs. CONCLUSIONS: This study demonstrates that ACE2 is highly expressed in HCECs and can be upregulated by stimulation with inflammatory cytokines and inflamed mouse corneal epithelium. Resveratrol may be able to reduce the increased expression of ACE2 on the inflammatory ocular surface. Our work suggests that patients with an inflammatory ocular surface may display higher ACE2 expression, which increases the risk of SARS-CoV-2 infection. |Adult[MESH] |Angiotensin-Converting Enzyme 2/*genetics/metabolism[MESH] |Animals[MESH] |Blotting, Western[MESH] |Cells, Cultured[MESH] |Enzyme Inhibitors/*pharmacology[MESH] |Epithelium, Corneal/drug effects/*enzymology[MESH] |Gene Expression Regulation, Enzymologic/drug effects/*physiology[MESH] |Humans[MESH] |Inflammation/drug therapy/enzymology[MESH] |Interleukin-1beta/pharmacology[MESH] |Keratitis/drug therapy/*enzymology[MESH] |Male[MESH] |Mice[MESH] |Mice, Inbred BALB C[MESH] |Mice, Inbred C57BL[MESH] |Microscopy, Fluorescence[MESH] |RNA, Messenger/genetics[MESH] |Real-Time Polymerase Chain Reaction[MESH] |Receptors, Virus/metabolism[MESH] |Resveratrol/*pharmacology[MESH] |SARS-CoV-2/*physiology[MESH] |Serine Endopeptidases/genetics/metabolism[MESH] |Tumor Necrosis Factor-alpha/pharmacology[MESH]ACE2 Expression Is Upregulated in Inflammatory Corneal Epithelial Cell(epmc).pdf DeepDyve Pubget Overpricing