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10.1038/s41594-021-00619-0 http://scihub22266oqcxt.onion/10.1038/s41594-021-00619-0 34158638!8772433!34158638     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Struct+Mol+Biol 2021 ; 28 (7): 573-582 Nephropedia Template TP {{tp|p=34158638|t=2021. Cryo-EM structure of SARS-CoV-2 ORF3a in lipid nanodiscs |pdf=|usr=}}{{34158638}} gab.com Text Cryo-EM structure of SARS-CoV-2 ORF3a in lipid nanodiscs JO: Nat Struct Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=34158638 #FOAvip SARS-CoV-2 ORF3a is a putative viral ion channel implicated in autophagy inhibition, inflammasome activation and apoptosis. 3a protein and anti-3a antibodies are found in infected patient tissues and plasma. Deletion of 3a in SARS-CoV-1 reduces viral titer and morbidity in mice, suggesting it could be an effective target for vaccines or therapeutics. Here, we present structures of SARS-CoV-2 3a determined by cryo-EM to 2.1-A resolution. 3a adopts a new fold with a polar cavity that opens to the cytosol and membrane through separate water- and lipid-filled openings. Hydrophilic grooves along outer helices could form ion-conduction paths. Using electrophysiology and fluorescent ion imaging of 3a-reconstituted liposomes, we observe Ca(2+)-permeable, nonselective cation channel activity, identify mutations that alter ion permeability and discover polycationic inhibitors of 3a activity. 3a-like proteins are found across coronavirus lineages that infect bats and humans, suggesting that 3a-targeted approaches could treat COVID-19 and other coronavirus diseases. Twit Text FOAVip Cryo-EM structure of SARS-CoV-2 ORF3a in lipid nanodiscs ????Nat Struct Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=34158638 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34158638 #FOAvip English Wikipedia <ref>{{Cite pmid|34158638}}</ref> Cryo-EM structure of SARS-CoV-2 ORF3a in lipid nanodiscs #MMPMID34158638 Kern DM; Sorum B; Mali SS; Hoel CM; Sridharan S; Remis JP; Toso DB; Kotecha A; Bautista DM; Brohawn SG Nat Struct Mol Biol 2021[Jul]; 28 (7): 573-582 PMID34158638show ga SARS-CoV-2 ORF3a is a putative viral ion channel implicated in autophagy inhibition, inflammasome activation and apoptosis. 3a protein and anti-3a antibodies are found in infected patient tissues and plasma. Deletion of 3a in SARS-CoV-1 reduces viral titer and morbidity in mice, suggesting it could be an effective target for vaccines or therapeutics. Here, we present structures of SARS-CoV-2 3a determined by cryo-EM to 2.1-A resolution. 3a adopts a new fold with a polar cavity that opens to the cytosol and membrane through separate water- and lipid-filled openings. Hydrophilic grooves along outer helices could form ion-conduction paths. Using electrophysiology and fluorescent ion imaging of 3a-reconstituted liposomes, we observe Ca(2+)-permeable, nonselective cation channel activity, identify mutations that alter ion permeability and discover polycationic inhibitors of 3a activity. 3a-like proteins are found across coronavirus lineages that infect bats and humans, suggesting that 3a-targeted approaches could treat COVID-19 and other coronavirus diseases. |*Cryoelectron Microscopy[MESH] |*Nanostructures/chemistry/ultrastructure[MESH] |*SARS-CoV-2/chemistry/ultrastructure[MESH] |Animals[MESH] |Calcium/metabolism[MESH] |Chiroptera/virology[MESH] |Coronaviridae[MESH] |Electrophysiology[MESH] |Fluorescence[MESH] |Humans[MESH] |Ion Transport[MESH] |Liposomes[MESH] |Models, Molecular[MESH] |Open Reading Frames[MESH] |Optical Imaging[MESH] |Reproducibility of Results[MESH] |Sequence Homology[MESH] |Viral Proteins/chemistry/ultrastructure[MESH]Cryo-EM structure of SARS-CoV-2 ORF3a in lipid nanodiscs (epmc).pdf DeepDyve Pubget Overpricing