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10.1093/jalm/jfab066

http://scihub22266oqcxt.onion/10.1093/jalm/jfab066
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34156472!8344811!34156472
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suck abstract from ncbi


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pmid34156472      J+Appl+Lab+Med 2021 ; 6 (6): 1635-1639
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  • Comparison of Three Sample-to-Answer RT-PCR Testing Platforms for the Detection of SARS-CoV-2 RNA in Positive Nasopharyngeal and Nasal Swabs #MMPMID34156472
  • Luethy PM; Johnson JK
  • J Appl Lab Med 2021[Nov]; 6 (6): 1635-1639 PMID34156472show ga
  • BACKGROUND: The COVID-19 pandemic has strained clinical microbiology laboratories due to testing supply allocations. As a result, laboratories have had to invest in multiple COVID-19 assays performed on different testing instruments. Comparing the results achieved by testing positive samples between in-use assays can provide insights into which platforms may be interchangeable for testing in times of supply chain emergencies. METHODS: Nasopharyngeal and nasal swab specimens collected in viral transport media that tested positive on the Xpert(R) Xpress SARS-CoV-2 assay were tested on the ePlex(R) SARS-CoV-2 and BD SARS-CoV-2 Reagents for BD Max assays. Positive percent agreement was calculated using the Xpert Xpress SARS-CoV-2 assay as the reference method. RESULTS: We tested 78 positive swabs, resulting in a positive percentage agreement (PPA) of 92% (CI 84-97%) for the BD SARS-CoV-2 assay and 58% (CI 47-70%) for the ePlex assay. Following development of a new workflow for the ePlex, we detected SARS-CoV-2 in 7 additional samples, resulting in a new PPA of 68% (CI 56-78). CONCLUSIONS: During times of supply allocation and shortage of the Xpert Xpress SARS-CoV-2 assay, the BD SARS-CoV-2 assay is well suited to test substitutions due to its high PPA.
  • |*COVID-19[MESH]
  • |*Pandemics[MESH]
  • |Humans[MESH]
  • |RNA, Viral/genetics[MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction[MESH]
  • |SARS-CoV-2[MESH]


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