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10.1248/bpb.b21-00387 http://scihub22266oqcxt.onion/10.1248/bpb.b21-00387 34148926!ä!34148926 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34148926&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biol+Pharm+Bull 2021 ; 44 (9): 1332-1336 Nephropedia Template TP {{tp|p=34148926|t=2021. Improved Detection Sensitivity of an Antigen Test for SARS-CoV-2 Nucleocapsid Proteins with Thio-NAD Cycling |pdf=|usr=}}{{34148926}} gab.com Text Improved Detection Sensitivity of an Antigen Test for SARS-CoV-2 Nucleocapsid Proteins with Thio-NAD Cycling JO: Biol Pharm Bull http://www.kidney.de/pget.php?&tw=1&pmid=34148926 #FOAvip Antigen tests for infectious diseases are inexpensive and easy-to-use, but the limit of detection (LOD) is generally higher than that of PCR tests, which are considered the gold standard. In the present study, we combined a sandwich enzyme-linked immunosorbent assay (ELISA) with thionicotinamide-adenine dinucleotide (thio-NAD) cycling to improve the LOD of antigen tests for coronavirus disease 2019 (COVID-19). For recombinant nucleocapsid proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the LOD of our ELISA with thio-NAD cycling was 2.95 x 10(-17) moles/assay. When UV-irradiated inactive SARS-CoV-2 was used, the minimum detectable virions corresponding to 2.6 x 10(4) RNA copies/assay were obtained using our ELISA with thio-NAD cycling. The assay volume for each test was 100 microL. The minimum detectable value was smaller than that of the latest antigen test using a fluorescent immunoassay for SARS-CoV-2, indicating the validity of our detection system for COVID-19 diagnosis. Twit Text FOAVip Improved Detection Sensitivity of an Antigen Test for SARS-CoV-2 Nucleocapsid Proteins with Thio-NAD Cycling ????Biol Pharm Bull http://www.kidney.de/pget.php?&tw=1&pmid=34148926 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34148926 #FOAvip English Wikipedia <ref>{{Cite pmid|34148926}}</ref> Improved Detection Sensitivity of an Antigen Test for SARS-CoV-2 Nucleocapsid Proteins with Thio-NAD Cycling #MMPMID34148926 Kyosei Y; Namba M; Yamura S; Watabe S; Yoshimura T; Sasaki T; Shioda T; Ito E Biol Pharm Bull 2021[Sep]; 44 (9): 1332-1336 PMID34148926show ga Antigen tests for infectious diseases are inexpensive and easy-to-use, but the limit of detection (LOD) is generally higher than that of PCR tests, which are considered the gold standard. In the present study, we combined a sandwich enzyme-linked immunosorbent assay (ELISA) with thionicotinamide-adenine dinucleotide (thio-NAD) cycling to improve the LOD of antigen tests for coronavirus disease 2019 (COVID-19). For recombinant nucleocapsid proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the LOD of our ELISA with thio-NAD cycling was 2.95 x 10(-17) moles/assay. When UV-irradiated inactive SARS-CoV-2 was used, the minimum detectable virions corresponding to 2.6 x 10(4) RNA copies/assay were obtained using our ELISA with thio-NAD cycling. The assay volume for each test was 100 microL. The minimum detectable value was smaller than that of the latest antigen test using a fluorescent immunoassay for SARS-CoV-2, indicating the validity of our detection system for COVID-19 diagnosis. |*Antibodies, Viral[MESH] |*SARS-CoV-2[MESH] |Antigens, Viral[MESH] |COVID-19 Testing/*methods[MESH] |COVID-19/*diagnosis/virology[MESH] |Enzyme-Linked Immunosorbent Assay/*methods[MESH] |Humans[MESH] |Immunologic Tests[MESH] |Limit of Detection[MESH] |NAD/*analogs & derivatives[MESH] |Nucleocapsid Proteins/*immunology[MESH] |Nucleocapsid/analysis[MESH]Improved Detection Sensitivity of an Antigen Test for SARS-CoV-2 Nucle(epmc).pdf DeepDyve Pubget Overpricing