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10.1016/j.cca.2021.06.016 http://scihub22266oqcxt.onion/10.1016/j.cca.2021.06.016 34144041!8206622!34144041     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Clin+Chim+Acta 2021 ; 521 (ä): 9-18 Nephropedia Template TP {{tp|p=34144041|t=2021. Comparison of two digital PCR methods for EGFR DNA and SARS-CoV-2 RNA quantification |pdf=|usr=}}{{34144041}} gab.com Text Comparison of two digital PCR methods for EGFR DNA and SARS-CoV-2 RNA quantification JO: Clin Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=34144041 #FOAvip BACKGROUND: The COVID-19 pandemic caused by the severe acute SARS-CoV-2 virus has undeniably highlighted the importance of reliable nucleic acid quantification. Digital PCR (dPCR) is capable of the absolute quantification of nucleic acids. METHOD: By using the droplet dPCR (QX200) and the digital real-time PCR (LOAA), the copy numbers were compared via multiple assays for three distinct targerts; EGFR DNA, SARS-CoV-2 and HIV-1 RNA. RESULTS: The droplet dPCR and digital real-time PCR showed similar copy numbers for both DNA and RNA quantification. When the limit of detection (LOD) and limit of quantitation (LOQ) of each method were estimated for DNA and RNA targets, the digital real-time PCR showed a higher sensitivity and precision especially with low copy number targets. CONCLUSION: The breath of nucleic acid testing in diagnostic applications continues to expand. In this study we applied common diagnostic targets to a novel digital real-time PCR methodology. It performed comparably to the established dPCR method with distinctive advantages and disadvantages for implementing in laboratories. These rapidly developing dPCR systems can be applied to benefit the accurate and sensitive nucleic acid testing for various clinical areas. Twit Text FOAVip Comparison of two digital PCR methods for EGFR DNA and SARS-CoV-2 RNA quantification ????Clin Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=34144041 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34144041 #FOAvip English Wikipedia <ref>{{Cite pmid|34144041}}</ref> Comparison of two digital PCR methods for EGFR DNA and SARS-CoV-2 RNA quantification #MMPMID34144041 Lee SS; Park JH; Bae YK Clin Chim Acta 2021[Oct]; 521 (ä): 9-18 PMID34144041show ga BACKGROUND: The COVID-19 pandemic caused by the severe acute SARS-CoV-2 virus has undeniably highlighted the importance of reliable nucleic acid quantification. Digital PCR (dPCR) is capable of the absolute quantification of nucleic acids. METHOD: By using the droplet dPCR (QX200) and the digital real-time PCR (LOAA), the copy numbers were compared via multiple assays for three distinct targerts; EGFR DNA, SARS-CoV-2 and HIV-1 RNA. RESULTS: The droplet dPCR and digital real-time PCR showed similar copy numbers for both DNA and RNA quantification. When the limit of detection (LOD) and limit of quantitation (LOQ) of each method were estimated for DNA and RNA targets, the digital real-time PCR showed a higher sensitivity and precision especially with low copy number targets. CONCLUSION: The breath of nucleic acid testing in diagnostic applications continues to expand. In this study we applied common diagnostic targets to a novel digital real-time PCR methodology. It performed comparably to the established dPCR method with distinctive advantages and disadvantages for implementing in laboratories. These rapidly developing dPCR systems can be applied to benefit the accurate and sensitive nucleic acid testing for various clinical areas. |*COVID-19[MESH] |*SARS-CoV-2[MESH] |DNA[MESH] |ErbB Receptors[MESH] |Humans[MESH] |Pandemics[MESH] |RNA, Viral/genetics[MESH]Comparison of two digital PCR methods for EGFR DNA and SARS-CoV-2 RNA (epmc).pdf DeepDyve Pubget Overpricing