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10.1038/s41598-021-91809-9 http://scihub22266oqcxt.onion/10.1038/s41598-021-91809-9 34140558!8211782!34140558     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34140558&cmd=llinks): Failed to open stream: HTTP request failed! 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An ACE2 Triple Decoy that neutralizes SARS-CoV-2 shows enhanced affinity for virus variants |pdf=|usr=}}{{34140558}} gab.com Text An ACE2 Triple Decoy that neutralizes SARS-CoV-2 shows enhanced affinity for virus variants JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34140558 #FOAvip The SARS-CoV-2 variants replacing the first wave strain pose an increased threat by their potential ability to escape pre-existing humoral protection. An angiotensin converting enzyme 2 (ACE2) decoy that competes with endogenous ACE2 for binding of the SARS-CoV-2 spike receptor binding domain (S RBD) and inhibits infection may offer a therapeutic option with sustained efficacy against variants. Here, we used Molecular Dynamics (MD) simulation to predict ACE2 sequence substitutions that might increase its affinity for S RBD and screened candidate ACE2 decoys in vitro. The lead ACE2(T27Y/H34A)-IgG(1)F(C) fusion protein with enhanced S RBD affinity shows greater live SARS-CoV-2 virus neutralization capability than wild type ACE2. MD simulation was used to predict the effects of S RBD variant mutations on decoy affinity that was then confirmed by testing of an ACE2 Triple Decoy that included an additional enzyme activity-deactivating H374N substitution against mutated S RBD. The ACE2 Triple Decoy maintains high affinity for mutated S RBD, displays enhanced affinity for S RBD N501Y or L452R, and has the highest affinity for S RBD with both E484K and N501Y mutations, making it a viable therapeutic option for the prevention or treatment of SARS-CoV-2 infection with a high likelihood of efficacy against variants. Twit Text FOAVip An ACE2 Triple Decoy that neutralizes SARS-CoV-2 shows enhanced affinity for virus variants ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34140558 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34140558 #FOAvip English Wikipedia <ref>{{Cite pmid|34140558}}</ref> An ACE2 Triple Decoy that neutralizes SARS-CoV-2 shows enhanced affinity for virus variants #MMPMID34140558 Tanaka S; Nelson G; Olson CA; Buzko O; Higashide W; Shin A; Gonzalez M; Taft J; Patel R; Buta S; Richardson A; Bogunovic D; Spilman P; Niazi K; Rabizadeh S; Soon-Shiong P Sci Rep 2021[Jun]; 11 (1): 12740 PMID34140558show ga The SARS-CoV-2 variants replacing the first wave strain pose an increased threat by their potential ability to escape pre-existing humoral protection. An angiotensin converting enzyme 2 (ACE2) decoy that competes with endogenous ACE2 for binding of the SARS-CoV-2 spike receptor binding domain (S RBD) and inhibits infection may offer a therapeutic option with sustained efficacy against variants. Here, we used Molecular Dynamics (MD) simulation to predict ACE2 sequence substitutions that might increase its affinity for S RBD and screened candidate ACE2 decoys in vitro. The lead ACE2(T27Y/H34A)-IgG(1)F(C) fusion protein with enhanced S RBD affinity shows greater live SARS-CoV-2 virus neutralization capability than wild type ACE2. MD simulation was used to predict the effects of S RBD variant mutations on decoy affinity that was then confirmed by testing of an ACE2 Triple Decoy that included an additional enzyme activity-deactivating H374N substitution against mutated S RBD. The ACE2 Triple Decoy maintains high affinity for mutated S RBD, displays enhanced affinity for S RBD N501Y or L452R, and has the highest affinity for S RBD with both E484K and N501Y mutations, making it a viable therapeutic option for the prevention or treatment of SARS-CoV-2 infection with a high likelihood of efficacy against variants. |*Amino Acid Substitution[MESH] |*Molecular Dynamics Simulation[MESH] |Amino Acid Sequence[MESH] |Angiotensin-Converting Enzyme 2/*chemistry/*metabolism[MESH] |Antiviral Agents/*pharmacology[MESH] |COVID-19/*metabolism/virology[MESH] |Drug Discovery/*methods[MESH] |Humans[MESH] |Mutation[MESH] |Protein Binding/drug effects[MESH] |Protein Domains/genetics[MESH] |SARS-CoV-2/*metabolism[MESH] |Signal Transduction/*drug effects[MESH] |Spike Glycoprotein, Coronavirus/metabolism[MESH]An ACE2 Triple Decoy that neutralizes SARS-CoV-2 shows enhanced affini(epmc).pdf DeepDyve Pubget Overpricing