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10.1038/s41541-021-00346-z http://scihub22266oqcxt.onion/10.1038/s41541-021-00346-z 34140522!8211640!34140522     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34140522&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       NPJ+Vaccines 2021 ; 6 (1): 83 Nephropedia Template TP {{tp|p=34140522|t=2021. A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells |pdf=|usr=}}{{34140522}} gab.com Text A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells JO: NPJ Vaccines http://www.kidney.de/pget.php?&tw=1&pmid=34140522 #FOAvip An array of SARS-CoV-2 virus variants have been isolated, propagated and used in in vitro assays, in vivo animal studies and human clinical trials. Observations of working stocks of SARS-CoV-2 suggest that sequential propagation in Vero cells leads to critical changes in the region of the furin cleavage site, which significantly reduce the value of the working stock for critical research studies. Serially propagating SARS-CoV-2 in Vero E6 cells leads to rapid increases in genetic variants while propagation in other cell lines (e.g. Vero/hSLAM) appears to mitigate this risk thereby improving the overall genetic stability of working stocks. From these observations, investigators are urged to monitor genetic variants carefully when propagating SARS-CoV-2 in Vero cells. Twit Text FOAVip A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells ????NPJ Vaccines http://www.kidney.de/pget.php?&tw=1&pmid=34140522 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34140522 #FOAvip English Wikipedia <ref>{{Cite pmid|34140522}}</ref> A cautionary perspective regarding the isolation and serial propagation of SARS-CoV-2 in Vero cells #MMPMID34140522 Funnell SGP; Afrough B; Baczenas JJ; Berry N; Bewley KR; Bradford R; Florence C; Duff YL; Lewis M; Moriarty RV; Connor SLO; Osman KL; Pullan S; Rashid S; Richards KS; Stemple KJ; Knezevic I NPJ Vaccines 2021[Jun]; 6 (1): 83 PMID34140522show ga An array of SARS-CoV-2 virus variants have been isolated, propagated and used in in vitro assays, in vivo animal studies and human clinical trials. Observations of working stocks of SARS-CoV-2 suggest that sequential propagation in Vero cells leads to critical changes in the region of the furin cleavage site, which significantly reduce the value of the working stock for critical research studies. Serially propagating SARS-CoV-2 in Vero E6 cells leads to rapid increases in genetic variants while propagation in other cell lines (e.g. Vero/hSLAM) appears to mitigate this risk thereby improving the overall genetic stability of working stocks. From these observations, investigators are urged to monitor genetic variants carefully when propagating SARS-CoV-2 in Vero cells. ?A cautionary perspective regarding the isolation and serial propagatio(epmc).pdf DeepDyve Pubget Overpricing