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10.2144/btn-2021-0044

http://scihub22266oqcxt.onion/10.2144/btn-2021-0044
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34139879!8212844!34139879
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suck abstract from ncbi


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pmid34139879      Biotechniques 2021 ; 71 (1): 370-375
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  • Tergitol-15-S-9 inactivates SARS-CoV-2 and boosts immunoassay signals #MMPMID34139879
  • Berg MG; Israeli E; Quaco E; Cloherty GA; Hemken PM
  • Biotechniques 2021[Jul]; 71 (1): 370-375 PMID34139879show ga
  • Inactivation of SARS-CoV-2 virus is necessary to mitigate risk but may interfere with diagnostic assay performance. We examined the effect of heat inactivation on a prototype SARS-CoV-2 antigen immunoassay run on the ARCHITECT automated analyzer. Recombinant full-length SARS-CoV-2 nucleocapsid protein and virus lysate detection was reduced by 66 and 31%, respectively. Several nonionic detergents were assessed as inactivation alternatives based on infectivity in cultured Vero CCL81 cells. Incubation of SARS-CoV-2 in 0.1% Tergitol 15-S-9 for 10 min significantly reduced infectivity and increased the immunoassay signal for cultured lysate and patient specimens. Tergitol 15-S-9 can inactivate SARS-CoV-2 while preserving epitopes on the nucleocapsid protein for enhanced detection by immunoassay antibodies.
  • |Animals[MESH]
  • |Antibodies, Viral/drug effects/immunology[MESH]
  • |COVID-19 Testing/*methods/standards[MESH]
  • |COVID-19/diagnosis/immunology[MESH]
  • |Cells, Cultured[MESH]
  • |Chlorocebus aethiops[MESH]
  • |Humans[MESH]
  • |Immunoassay/methods/standards[MESH]
  • |Nucleocapsid/immunology[MESH]
  • |Poloxalene/*pharmacology[MESH]
  • |SARS-CoV-2/*drug effects/*immunology[MESH]
  • |Surface-Active Agents/pharmacology[MESH]
  • |Vero Cells[MESH]


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