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10.1016/j.mcpro.2021.100113 http://scihub22266oqcxt.onion/10.1016/j.mcpro.2021.100113 34139362!8205261!34139362     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34139362&cmd=llinks): Failed to open stream: HTTP request failed! 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Proteome Profiling of Recombinant DNase Therapy in Reducing NETs and Aiding Recovery in COVID-19 Patients |pdf=|usr=}}{{34139362}} gab.com Text Proteome Profiling of Recombinant DNase Therapy in Reducing NETs and Aiding Recovery in COVID-19 Patients JO: Mol Cell Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=34139362 #FOAvip Severe coronavirus disease 2019 (COVID-19) can result in pneumonia and acute respiratory failure. Accumulation of mucus in the airways is a hallmark of the disease and can result in hypoxemia. Here, we show that quantitative proteome analysis of the sputum from severe patients with COVID-19 reveal high levels of neutrophil extracellular trap (NET) components, which was confirmed by microscopy. Extracellular DNA from excessive NET formation can increase sputum viscosity and lead to acute respiratory distress syndrome. Recombinant human DNase (Pulmozyme; Roche) has been shown to be beneficial in reducing sputum viscosity and improve lung function. We treated five patients pwith COVID-19 resenting acute symptoms with clinically approved aerosolized Pulmozyme. No adverse reactions to the drug were seen, and improved oxygen saturation and recovery in all severely ill patients with COVID-19 was observed after therapy. Immunofluorescence and proteome analysis of sputum and blood plasma samples after treatment revealed a marked reduction of NETs and a set of statistically significant proteome changes that indicate reduction of hemorrhage, plasma leakage and inflammation in the airways, and reduced systemic inflammatory state in the blood plasma of patients. Taken together, the results indicate that NETs contribute to acute respiratory failure in COVID-19 and that degrading NETs may reduce dependency on external high-flow oxygen therapy in patients. Targeting NETs using recombinant human DNase may have significant therapeutic implications in COVID-19 disease and warrants further studies. Twit Text FOAVip Proteome Profiling of Recombinant DNase Therapy in Reducing NETs and Aiding Recovery in COVID-19 Patients ????Mol Cell Proteomics http://www.kidney.de/pget.php?&tw=1&pmid=34139362 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34139362 #FOAvip English Wikipedia <ref>{{Cite pmid|34139362}}</ref> Proteome Profiling of Recombinant DNase Therapy in Reducing NETs and Aiding Recovery in COVID-19 Patients #MMPMID34139362 Fisher J; Mohanty T; Karlsson CAQ; Khademi SMH; Malmstrom E; Frigyesi A; Nordenfelt P; Malmstrom J; Linder A Mol Cell Proteomics 2021[]; 20 (ä): 100113 PMID34139362show ga Severe coronavirus disease 2019 (COVID-19) can result in pneumonia and acute respiratory failure. Accumulation of mucus in the airways is a hallmark of the disease and can result in hypoxemia. Here, we show that quantitative proteome analysis of the sputum from severe patients with COVID-19 reveal high levels of neutrophil extracellular trap (NET) components, which was confirmed by microscopy. Extracellular DNA from excessive NET formation can increase sputum viscosity and lead to acute respiratory distress syndrome. Recombinant human DNase (Pulmozyme; Roche) has been shown to be beneficial in reducing sputum viscosity and improve lung function. We treated five patients pwith COVID-19 resenting acute symptoms with clinically approved aerosolized Pulmozyme. No adverse reactions to the drug were seen, and improved oxygen saturation and recovery in all severely ill patients with COVID-19 was observed after therapy. Immunofluorescence and proteome analysis of sputum and blood plasma samples after treatment revealed a marked reduction of NETs and a set of statistically significant proteome changes that indicate reduction of hemorrhage, plasma leakage and inflammation in the airways, and reduced systemic inflammatory state in the blood plasma of patients. Taken together, the results indicate that NETs contribute to acute respiratory failure in COVID-19 and that degrading NETs may reduce dependency on external high-flow oxygen therapy in patients. Targeting NETs using recombinant human DNase may have significant therapeutic implications in COVID-19 disease and warrants further studies. |*COVID-19 Drug Treatment[MESH] |Aged[MESH] |Blood Proteins/analysis[MESH] |COVID-19/metabolism/therapy[MESH] |Deoxyribonuclease I/*pharmacology[MESH] |Extracellular Traps/*metabolism[MESH] |Female[MESH] |Fluorescent Antibody Technique[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |Proteome/*analysis[MESH] |Recombinant Proteins/pharmacology[MESH] |Severity of Illness Index[MESH] |Sputum/drug effects/metabolism/virology[MESH]Proteome Profiling of Recombinant DNase Therapy in Reducing NETs and A(epmc).pdf DeepDyve Pubget Overpricing