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10.1016/j.bios.2021.113421 http://scihub22266oqcxt.onion/10.1016/j.bios.2021.113421 34134070!8186945!34134070     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biosens+Bioelectron 2021 ; 190 (ä): 113421 Nephropedia Template TP {{tp|p=34134070|t=2021. Ultrasensitive detection of SARS-CoV-2 spike protein in untreated saliva using SERS-based biosensor |pdf=|usr=}}{{34134070}} gab.com Text Ultrasensitive detection of SARS-CoV-2 spike protein in untreated saliva using SERS-based biosensor JO: Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=34134070 #FOAvip Early diagnosis and monitoring of SARS-CoV-2 virus is essential to control COVID-19 outbreak. In this study, we propose a promising surface enhanced Raman scattering (SERS)-based COVID-19 biosensor for ultrasensitive detection of SARS-CoV-2 virus in untreated saliva. The SERS-immune substrate was fabricated by a novel oil/water/oil (O/W/O) three-phase liquid-liquid interfaces self-assembly method, forming two layers of dense and uniform gold nanoparticle films to ensure the reproducibility and sensitivity of SERS immunoassay. The detection was performed by an immunoreaction between the SARS-CoV-2 spike antibody modified SERS-immune substrate, spike antigen protein and Raman reporter-labeled immuno-Ag nanoparticles. This SERS-based biosensor was able to detect the SARS-CoV-2 spike protein at concentrations of 0.77 fg mL(-1) in phosphate-buffered saline and 6.07 fg mL(-1) in untreated saliva. The designed SERS-based biosensor exhibited excellent specificity and sensitivity for SARS-CoV-2 virus without any sample pretreatment, providing a potential choice for the early diagnosis of COVID-19. Twit Text FOAVip Ultrasensitive detection of SARS-CoV-2 spike protein in untreated saliva using SERS-based biosensor ????Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=34134070 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34134070 #FOAvip English Wikipedia <ref>{{Cite pmid|34134070}}</ref> Ultrasensitive detection of SARS-CoV-2 spike protein in untreated saliva using SERS-based biosensor #MMPMID34134070 Zhang M; Li X; Pan J; Zhang Y; Zhang L; Wang C; Yan X; Liu X; Lu G Biosens Bioelectron 2021[Oct]; 190 (ä): 113421 PMID34134070show ga Early diagnosis and monitoring of SARS-CoV-2 virus is essential to control COVID-19 outbreak. In this study, we propose a promising surface enhanced Raman scattering (SERS)-based COVID-19 biosensor for ultrasensitive detection of SARS-CoV-2 virus in untreated saliva. The SERS-immune substrate was fabricated by a novel oil/water/oil (O/W/O) three-phase liquid-liquid interfaces self-assembly method, forming two layers of dense and uniform gold nanoparticle films to ensure the reproducibility and sensitivity of SERS immunoassay. The detection was performed by an immunoreaction between the SARS-CoV-2 spike antibody modified SERS-immune substrate, spike antigen protein and Raman reporter-labeled immuno-Ag nanoparticles. This SERS-based biosensor was able to detect the SARS-CoV-2 spike protein at concentrations of 0.77 fg mL(-1) in phosphate-buffered saline and 6.07 fg mL(-1) in untreated saliva. The designed SERS-based biosensor exhibited excellent specificity and sensitivity for SARS-CoV-2 virus without any sample pretreatment, providing a potential choice for the early diagnosis of COVID-19. |*Biosensing Techniques[MESH] |*COVID-19[MESH] |*Metal Nanoparticles[MESH] |Gold[MESH] |Humans[MESH] |Reproducibility of Results[MESH] |SARS-CoV-2[MESH] |Saliva[MESH]Ultrasensitive detection of SARS-CoV-2 spike protein in untreated sali(epmc).pdf DeepDyve Pubget Overpricing