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10.1016/j.jcv.2021.104877 http://scihub22266oqcxt.onion/10.1016/j.jcv.2021.104877 34134034!8164504!34134034     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Virol 2021 ; 141 (ä): 104877 Nephropedia Template TP {{tp|p=34134034|t=2021. Rapid and simultaneous identification of three mutations by the Novaplex SARS-CoV-2 variants I assay kit |pdf=|usr=}}{{34134034}} gab.com Text Rapid and simultaneous identification of three mutations by the Novaplex SARS-CoV-2 variants I assay kit JO: J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=34134034 #FOAvip BACKGROUND: . The emergence of SARS-CoV-2 variants has caused an unexpected rebound globally. The World Health Organization has listed three variants (B.1.1.7, B.1.351, and P.1) as variants of concern. To understand the epidemiology and thereby plan appropriate safety measures, differential identification of the variants is indeed critical. OBJECTIVES: . Although whole-genome sequencing is the gold standard for variant identification, it is time-consuming and relatively expensive. Therefore, a rapid, easy, and cost-effective platform targeting multiple regions of the genome is required. Here, we assessed the usefulness of the Novaplex SARS-CoV-2 Variants I Assay kit in identifying mutations in the variants. STUDY DESIGN: . We retrospectively examined 30 stored nasal swabs from COVID-19-positive patients tested between November 2020 and March 2021. RNA extracted from these swabs was subjected to the commercial kit and real-time reverse transcription-PCR was performed. To determine the genome sequences of SARS-CoV-2 in the collected samples and deduce the consensus sequences among the identified variants, genome sequencing libraries were prepared and mapped to the reference genome. RESULTS: . Four of the tested samples were determined as variants. Of them, two harbored both H69/V70 deletion and N501Y substitution, whereas two harbored E484K substitution alone. CONCLUSIONS: . The variant with E484K substitution alone ("R.1") has been now categorized as a variant of interest in Japan. Additionally, the kit-based assay was found to be feasible, convenient, and user-friendly in identifying the abovementioned mutations with a turnaround time of only 2 h. Twit Text FOAVip Rapid and simultaneous identification of three mutations by the Novaplex SARS-CoV-2 variants I assay kit ????J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=34134034 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34134034 #FOAvip English Wikipedia <ref>{{Cite pmid|34134034}}</ref> Rapid and simultaneous identification of three mutations by the Novaplex SARS-CoV-2 variants I assay kit #MMPMID34134034 Kami W; Kinjo T; Arakaki W; Oki H; Motooka D; Nakamura S; Fujita J J Clin Virol 2021[Aug]; 141 (ä): 104877 PMID34134034show ga BACKGROUND: . The emergence of SARS-CoV-2 variants has caused an unexpected rebound globally. The World Health Organization has listed three variants (B.1.1.7, B.1.351, and P.1) as variants of concern. To understand the epidemiology and thereby plan appropriate safety measures, differential identification of the variants is indeed critical. OBJECTIVES: . Although whole-genome sequencing is the gold standard for variant identification, it is time-consuming and relatively expensive. Therefore, a rapid, easy, and cost-effective platform targeting multiple regions of the genome is required. Here, we assessed the usefulness of the Novaplex SARS-CoV-2 Variants I Assay kit in identifying mutations in the variants. STUDY DESIGN: . We retrospectively examined 30 stored nasal swabs from COVID-19-positive patients tested between November 2020 and March 2021. RNA extracted from these swabs was subjected to the commercial kit and real-time reverse transcription-PCR was performed. To determine the genome sequences of SARS-CoV-2 in the collected samples and deduce the consensus sequences among the identified variants, genome sequencing libraries were prepared and mapped to the reference genome. RESULTS: . Four of the tested samples were determined as variants. Of them, two harbored both H69/V70 deletion and N501Y substitution, whereas two harbored E484K substitution alone. CONCLUSIONS: . The variant with E484K substitution alone ("R.1") has been now categorized as a variant of interest in Japan. Additionally, the kit-based assay was found to be feasible, convenient, and user-friendly in identifying the abovementioned mutations with a turnaround time of only 2 h. |*COVID-19[MESH] |*SARS-CoV-2[MESH] |Humans[MESH] |Mutation[MESH] |Retrospective Studies[MESH]Rapid and simultaneous identification of three mutations by the Novapl(epmc).pdf DeepDyve Pubget Overpricing