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10.3892/mmr.2021.12223 http://scihub22266oqcxt.onion/10.3892/mmr.2021.12223 34132373!ä!34132373 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Med+Rep 2021 ; 24 (2): ä Nephropedia Template TP {{tp|p=34132373|t=2021. SARS?CoV?2 spike protein?induced host inflammatory response signature in human corneal epithelial cells |pdf=|usr=}}{{34132373}} gab.com Text SARS CoV 2 spike protein induced host inflammatory response signature in human corneal epithelial cells JO: Mol Med Rep http://www.kidney.de/pget.php?&tw=1&pmid=34132373 #FOAvip Coronavirus disease 2019 (COVID?19), caused by the severe acute respiratory syndrome coronavirus?2 (SARS?CoV?2), led to an outbreak of viral pneumonia in December 2019. The present study aimed to investigate the host inflammatory response signature?caused by SARS?CoV?2 in human corneal epithelial cells (HCECs). The expression level of angiotensin?converting enzyme 2 (ACE2) in the human cornea was determined via immunofluorescence. In vitro experiments were performed in HCECs stimulated with the SARS?CoV?2 spike protein. Moreover, the expression levels of ACE2, IL?8, TNF?alpha, IL?6, gasdermin D (GSDMD) and IL?1beta in HCECs were detected using reverse transcription?quantitative PCR and/or western blotting. It was identified that ACE2 was expressed in normal human corneal epithelium and HCECs cultured in vitro. Furthermore, the expression levels of IL?8, TNF?alpha and IL?6 in HCECs were decreased following SARS?CoV?2 spike protein stimulation, while the expression levels of GSDMD and IL?1beta were increased. In conclusion, the present results demonstrated that the SARS?CoV?2 spike protein suppressed the host inflammatory response and induced pyroptosis in HCECs. Therefore, blocking the ACE2 receptor in HCECs may reduce the infection rate of COVID?19. Twit Text FOAVip SARS CoV 2 spike protein induced host inflammatory response signature in human corneal epithelial cells ????Mol Med Rep http://www.kidney.de/pget.php?&tw=1&pmid=34132373 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34132373 #FOAvip English Wikipedia <ref>{{Cite pmid|34132373}}</ref> SARS?CoV?2 spike protein?induced host inflammatory response signature in human corneal epithelial cells #MMPMID34132373 Zhu G; Lyu L; Yang H; Liu G; Yang S; Gu C; Wang L; Yan H; Hu M; Che C Mol Med Rep 2021[Aug]; 24 (2): ä PMID34132373show ga Coronavirus disease 2019 (COVID?19), caused by the severe acute respiratory syndrome coronavirus?2 (SARS?CoV?2), led to an outbreak of viral pneumonia in December 2019. The present study aimed to investigate the host inflammatory response signature?caused by SARS?CoV?2 in human corneal epithelial cells (HCECs). The expression level of angiotensin?converting enzyme 2 (ACE2) in the human cornea was determined via immunofluorescence. In vitro experiments were performed in HCECs stimulated with the SARS?CoV?2 spike protein. Moreover, the expression levels of ACE2, IL?8, TNF?alpha, IL?6, gasdermin D (GSDMD) and IL?1beta in HCECs were detected using reverse transcription?quantitative PCR and/or western blotting. It was identified that ACE2 was expressed in normal human corneal epithelium and HCECs cultured in vitro. Furthermore, the expression levels of IL?8, TNF?alpha and IL?6 in HCECs were decreased following SARS?CoV?2 spike protein stimulation, while the expression levels of GSDMD and IL?1beta were increased. In conclusion, the present results demonstrated that the SARS?CoV?2 spike protein suppressed the host inflammatory response and induced pyroptosis in HCECs. Therefore, blocking the ACE2 receptor in HCECs may reduce the infection rate of COVID?19. |Adult[MESH] |Aged[MESH] |Angiotensin-Converting Enzyme 2/genetics/metabolism[MESH] |Cells, Cultured[MESH] |Cornea/cytology[MESH] |Epithelial Cells/cytology/metabolism/virology[MESH] |Epithelium, Corneal/*metabolism/virology[MESH] |Female[MESH] |Humans[MESH] |Interleukin-1beta/genetics/metabolism[MESH] |Interleukin-6/genetics/metabolism[MESH] |Intracellular Signaling Peptides and Proteins/genetics/metabolism[MESH] |Male[MESH] |Middle Aged[MESH] |Phosphate-Binding Proteins/genetics/metabolism[MESH] |Pyroptosis[MESH] |Spike Glycoprotein, Coronavirus/genetics/*metabolism[MESH] |Tumor Necrosis Factor-alpha/genetics/metabolism[MESH]SARS?CoV?2 spike protein?induced host inflammatory response signature (epmc).pdf DeepDyve Pubget Overpricing