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10.1101/2021.06.09.447760 http://scihub22266oqcxt.onion/10.1101/2021.06.09.447760 34127974!8202426!34127974     free     free     free       bioRxiv 2022 ; ? (?): ? Nephropedia Template TP {{tp|p=34127974|t=2022. Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection |pdf=|usr=}}{{34127974}} gab.com Text Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection JO: bioRxiv http://www.kidney.de/pget.php?&tw=1&pmid=34127974 #FOAvip The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the third human coronavirus within 20 years that gave rise to a life-threatening disease and the first to reach pandemic spread. To make therapeutic headway against current and future coronaviruses, the biology of coronavirus RNA during infection must be precisely understood. Here, we present a robust and generalizable framework combining high-throughput confocal and super-resolution microscopy imaging to study coronavirus infection at the nanoscale. Employing the model human coronavirus HCoV-229E, we specifically labeled coronavirus genomic RNA (gRNA) and double-stranded RNA (dsRNA) via multicolor RNA-immunoFISH and visualized their localization patterns within the cell. The exquisite resolution of our approach uncovers a striking spatial organization of gRNA and dsRNA into three distinct structures and enables quantitative characterization of the status of the infection after antiviral drug treatment. Our approach provides a comprehensive framework that supports investigations of coronavirus fundamental biology and therapeutic effects. Twit Text FOAVip Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection ????bioRxiv http://www.kidney.de/pget.php?&tw=1&pmid=34127974 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34127974 #FOAvip English Wikipedia <ref>{{Cite pmid|34127974}}</ref> Multi-color super-resolution imaging to study human coronavirus RNA during cellular infection #MMPMID34127974 Wang J; Han M; Roy AR; Wang H; Mockl L; Zeng L; Moerner WE; Qi LS bioRxiv 2022[Jan]; ? (?): ? PMID34127974show ga The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the third human coronavirus within 20 years that gave rise to a life-threatening disease and the first to reach pandemic spread. To make therapeutic headway against current and future coronaviruses, the biology of coronavirus RNA during infection must be precisely understood. Here, we present a robust and generalizable framework combining high-throughput confocal and super-resolution microscopy imaging to study coronavirus infection at the nanoscale. Employing the model human coronavirus HCoV-229E, we specifically labeled coronavirus genomic RNA (gRNA) and double-stranded RNA (dsRNA) via multicolor RNA-immunoFISH and visualized their localization patterns within the cell. The exquisite resolution of our approach uncovers a striking spatial organization of gRNA and dsRNA into three distinct structures and enables quantitative characterization of the status of the infection after antiviral drug treatment. Our approach provides a comprehensive framework that supports investigations of coronavirus fundamental biology and therapeutic effects. ?Multi-color super-resolution imaging to study human coronavirus RNA du(epmc).pdf DeepDyve Pubget Overpricing