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10.1101/2021.06.10.447768 http://scihub22266oqcxt.onion/10.1101/2021.06.10.447768 34127970!8202422!34127970     free     free     free       bioRxiv 2021 ; ä (ä): ä Nephropedia Template TP {{tp|p=34127970|t=2021. Identification of ACE2 modifiers by CRISPR screening |pdf=|usr=}}{{34127970}} gab.com Text Identification of ACE2 modifiers by CRISPR screening JO: bioRxiv http://www.kidney.de/pget.php?&tw=1&pmid=34127970 #FOAvip SARS-CoV-2 infection is initiated by binding of the viral spike protein to its receptor, ACE2, on the surface of host cells. ACE2 expression is heterogeneous both in vivo and in immortalized cell lines, but the molecular pathways that govern ACE2 expression remain unclear. We now report high-throughput CRISPR screens for functional modifiers of ACE2 surface abundance. We identified 35 genes whose disruption was associated with a change in the surface abundance of ACE2 in HuH7 cells. Enriched among these ACE2 regulators were established transcription factors, epigenetic regulators, and functional networks. We further characterized individual cell lines with disruption of SMAD4, EP300, PIAS1 , or BAMBI and found these genes to regulate ACE2 at the mRNA level and to influence cellular susceptibility to SARS-CoV-2 infection. Collectively, our findings clarify the host factors involved in SARS-CoV-2 entry and suggest potential targets for therapeutic development. Twit Text FOAVip Identification of ACE2 modifiers by CRISPR screening ????bioRxiv http://www.kidney.de/pget.php?&tw=1&pmid=34127970 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34127970 #FOAvip English Wikipedia <ref>{{Cite pmid|34127970}}</ref> Identification of ACE2 modifiers by CRISPR screening #MMPMID34127970 Sherman EJ; Mirabelli C; Tang VT; Khan TG; Kennedy AA; Graham SE; Willer CJ; Tai AW; Sexton JZ; Wobus CE; Emmer BT bioRxiv 2021[Jun]; ä (ä): ä PMID34127970show ga SARS-CoV-2 infection is initiated by binding of the viral spike protein to its receptor, ACE2, on the surface of host cells. ACE2 expression is heterogeneous both in vivo and in immortalized cell lines, but the molecular pathways that govern ACE2 expression remain unclear. We now report high-throughput CRISPR screens for functional modifiers of ACE2 surface abundance. We identified 35 genes whose disruption was associated with a change in the surface abundance of ACE2 in HuH7 cells. Enriched among these ACE2 regulators were established transcription factors, epigenetic regulators, and functional networks. We further characterized individual cell lines with disruption of SMAD4, EP300, PIAS1 , or BAMBI and found these genes to regulate ACE2 at the mRNA level and to influence cellular susceptibility to SARS-CoV-2 infection. Collectively, our findings clarify the host factors involved in SARS-CoV-2 entry and suggest potential targets for therapeutic development. äIdentification of ACE2 modifiers by CRISPR screening (epmc).pdf DeepDyve Pubget Overpricing