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10.1016/j.xpro.2021.100635 http://scihub22266oqcxt.onion/10.1016/j.xpro.2021.100635 34124695!8188458!34124695     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       STAR+Protoc 2021 ; 2 (3): 100635 Nephropedia Template TP {{tp|p=34124695|t=2021. Protein purification and crystallization of HLA-A *02:01 in complex with SARS-CoV-2 peptides |pdf=|usr=}}{{34124695}} gab.com Text Protein purification and crystallization of HLA-A *02:01 in complex with SARS-CoV-2 peptides JO: STAR Protoc http://www.kidney.de/pget.php?&tw=1&pmid=34124695 #FOAvip Understanding T-cell responses requires identifying viral peptides presented by human leukocyte antigens (HLAs). X-ray crystallography can be used to visualize their presentation. This protocol describes the expression, purification, and crystallization of HLA-A *02:01, one of the most frequent HLA in the global population in complex with peptides derived from the SARS-CoV-2 nucleocapsid protein. This protocol can be applied to different HLA class I molecules bound to other peptides. For complete details on the use and execution of this protocol, please refer to Szeto et al. (2021). Twit Text FOAVip Protein purification and crystallization of HLA-A *02:01 in complex with SARS-CoV-2 peptides ????STAR Protoc http://www.kidney.de/pget.php?&tw=1&pmid=34124695 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34124695 #FOAvip English Wikipedia <ref>{{Cite pmid|34124695}}</ref> Protein purification and crystallization of HLA-A *02:01 in complex with SARS-CoV-2 peptides #MMPMID34124695 Chatzileontiadou DSM; Szeto C; Jayasinghe D; Gras S STAR Protoc 2021[Sep]; 2 (3): 100635 PMID34124695show ga Understanding T-cell responses requires identifying viral peptides presented by human leukocyte antigens (HLAs). X-ray crystallography can be used to visualize their presentation. This protocol describes the expression, purification, and crystallization of HLA-A *02:01, one of the most frequent HLA in the global population in complex with peptides derived from the SARS-CoV-2 nucleocapsid protein. This protocol can be applied to different HLA class I molecules bound to other peptides. For complete details on the use and execution of this protocol, please refer to Szeto et al. (2021). |COVID-19/immunology/*metabolism/virology[MESH] |Coronavirus Nucleocapsid Proteins/*chemistry/isolation & purification/metabolism[MESH] |Crystallography, X-Ray[MESH] |Epitopes, T-Lymphocyte/immunology[MESH] |HLA-A2 Antigen/*chemistry/metabolism[MESH] |Humans[MESH] |Peptide Fragments/*chemistry/isolation & purification/metabolism[MESH] |Phosphoproteins/chemistry/isolation & purification/metabolism[MESH] |SARS-CoV-2/*metabolism[MESH]Protein purification and crystallization of HLA-A *02:01 in complex wi(epmc).pdf DeepDyve Pubget Overpricing